Volume 13, Issue 5 (September & October 2022)
BCN 2022, 13(5): 625-636 |
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Moayeri A, Alizadeh R, Ghasemi Hamidabadi H, Nazm Bojnordi M, Niapour A, Hedayatpour A, et al . Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture. BCN 2022; 13 (5) :625-636
URL: http://bcn.iums.ac.ir/article-1-1873-en.html
URL: http://bcn.iums.ac.ir/article-1-1873-en.html
Ardeshir Moayeri1 
, Rafieh Alizadeh2 , Hatef Ghasemi Hamidabadi * 3, Maryam Nazm Bojnordi3 , Ali Niapour4 , Azim Hedayatpour5 , Marzieh Darvishi1 , Fatemeh Heidari6 , Maryam Soleimani7 , Leila Elyasi8
, Rafieh Alizadeh2 , Hatef Ghasemi Hamidabadi * 3, Maryam Nazm Bojnordi3 , Ali Niapour4 , Azim Hedayatpour5 , Marzieh Darvishi1 , Fatemeh Heidari6 , Maryam Soleimani7 , Leila Elyasi8
1- Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
2- ENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Department of Anatomy & Cell Biology, Immunogenetic Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
4- Department of Anatomical Sciences, Research Laboratory for Embryology and Stem Cells, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
5- Department of Anatomy, School of Medicine, University of Tehran, Tehran, Iran.
6- Department of Anatomy, School of Medicine, Qom University of Medical Sciences, Qom, Iran.
7- Department of Basic Sciences, School of Rehabilitation Sciences, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
8- Department of Anatomy, Neuroscience Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
2- ENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Department of Anatomy & Cell Biology, Immunogenetic Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
4- Department of Anatomical Sciences, Research Laboratory for Embryology and Stem Cells, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
5- Department of Anatomy, School of Medicine, University of Tehran, Tehran, Iran.
6- Department of Anatomy, School of Medicine, Qom University of Medical Sciences, Qom, Iran.
7- Department of Basic Sciences, School of Rehabilitation Sciences, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
8- Department of Anatomy, Neuroscience Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Abstract:
Introduction: The induction of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases, including Parkinson disease. This study aims to differentiate HUC-MSCs into dopaminergic neuron-like cells.
Methods: Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography.
Results: Our results showed that dopaminergic neuronal markers’ transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates.
Conclusion: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.
Methods: Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography.
Results: Our results showed that dopaminergic neuronal markers’ transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates.
Conclusion: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.
Keywords: Differentiation, Dopaminergic neurons, Human umbilical cord mesenchymal stem cells, In vitro
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2020/07/27 | Accepted: 2021/03/7 | Published: 2022/09/11
Received: 2020/07/27 | Accepted: 2021/03/7 | Published: 2022/09/11
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