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1- Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
2- ENT and Head & Neck Research Center and Department, The Five Senses Institute, Iran University of Medical Sciences, Tehran, Iran.
3- Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
4- Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
5- Deptartment of Anatomy, School of Medicine, Medical Sciences/University of Tehran, Tehran, Iran.
6- Anatomy Department, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
7- Department of Basic Sciences, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
8- Neuroscience Research Center, Department of Anatomy, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Abstract:  
Introduction: The induction of from human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in   tissue engineering and experimental and clinical treatments of various neurodegenerative diseases including Parkinson’s disease. This study aimed to differentiate HUC-MSCs into dopaminergic neuron-like cells.
Methods: After HUC-MSCs isolation and characterizations, they transferred onto matrigel- coated plates and incubated with a  cocktail of dopaminergic neuronal differentiation factors. The capacity of  differentiation into dopaminergic neuron-like cells in 2D culture and on matrigel was assessed by real-time PCR, immunocytochemistry and high-performance liquid chromatography (HPLC).
Results: Our results showed that the transcript and protein levels of dopaminergic neuronal markers was significantly increased on  Matrigel differentiated cells as compared with  2D culture plates.
Conclusions: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potentials for the treatment of dopaminergic neuron-related diseases.
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2020/07/27 | Accepted: 2021/03/7

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