Highlights 
• Functional or sensory loss may follow peripheral nerve injury. 
• Surgical techniques and stimulating nerve growth are necessary to protect nerve regeneration. 
• The oriented basement membrane and extracellular matrix component of the autologous degenerated skeletal muscle could conduit the regenerating nerve fibers.  
• Nerve growth factor (NGF) has a vital role in the nerve growth and survival of neurons and increase nerve regeneration and remyelination after nerve defect.
• Combination of autologous degenerated skeletal muscle with NGF and laminin has promising treatment in the peripheral nerve injury

Plain Language Summary 
Peripheral nerve injury results in disability, limitation of daily living, and higher expenses for the family. This lesion requires a comprehensive treatment that provides both a conduit for nerve conduction and essential materials for nerve growth. Epimysium around the muscle fiber and epineurium around the nerve fiber have the same structure. Both of them are necessary for nerve and muscle survival and their function.  In this study, the muscle fiber was removed from the autologous degenerated skeletal muscle and basement membrane, and epimysium was used as a conduit for the interrupted nerve. NGF is a neuropeptide that participated in the regulation of nerve growth, adhesion, migration, neuronal proliferation, and regeneration and remyelination of nerve fiber. Laminin is a component of the basement membrane and act as a surface substrate for nerve repair and produced mainly by Schwann cells. Histological of the distal segment after the transplantation in the region demonstrated that axons could grow and reach the distal part. The study results indicate autologous degenerated skeletal muscle along with NGF and Laminin could provide a useful environment for axonal regeneration and functional recovery.