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Yasuj University of Medical Sciences, Yasuj, Iran
Abstract:  

Introduction: Peripheral nerve injury is one of the most common damages that lead to physical disability. Due to the similarity between the coatings of skeletal muscles with nerves fiber, this research conducted to determine the effect of muscle graft with nerve growth factor (NGF) and laminin (L) on nerve repair.
Methods: 10 mm sciatic nerve was transected and removed in forty–two female Wistar rats (200±250 g) and equally divided into three groups. In the muscle graft +NGF + laminin group degenerated skeletal muscle was sutured with proximal and distal ends of the transected sciatic nerve. Then, NGF (100 ng) and laminin (1.28 mg/ml) were injected into the muscle graft. In the muscle graft group, normal saline were injected into the muscle graft. In the control group the 10 mm sciatic nerve removed without any treatment. Functional recovery was assessed based on sciatic functional index (SFI), tracing and histological study was achieved to assess nerve repair.  
Results: The Mean SFI significantly increased in the muscle graft +NGF + laminin (-76.6±2.9) and muscle graft (-82.1±3.5) groups in the 60 days post injury compared to the control group (p<0.05). The mean number of Labeled motor neuron significantly increased in the muscle graft +NGF + laminin (78.6±3.1) and muscle graft (61.3±6.1) groups compared to the control group (p<0.001). Mean number of myelinated axons in the distal segments of the muscle graft +NGF + laminin increased significantly compare to the muscle graft group (p<0.05).
Conclusion: These findings suggest that muscle graft accompanying exogenous NGF and laminin provide clear therapeutic effects on nerve repair.

Type of Study: Original | Subject: Behavioral Neuroscience
Received: 2017/04/13 | Accepted: 2018/03/3 | Published: 2018/05/13

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