Volume 12, Issue 3 (May & June 2021)
BCN 2021, 12(3): 373-382 |
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Esfandiarifar A, Azarbayjani M A, Peeri M, Jameie S B. The Effect of Resistance Training and Berberine Chloride on the Apoptosis-related Unfolded Protein Response Signaling Pathway in the Hippocampus of Diazinon-poisoned Rats. BCN 2021; 12 (3) :373-382
URL: http://bcn.iums.ac.ir/article-1-1665-en.html
URL: http://bcn.iums.ac.ir/article-1-1665-en.html
1- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran.
2- Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran.
Abstract:
Introduction: Diazinon is one of the most widely-used organophosphate pesticides in the world. This toxin enters the body in various ways and induces oxidative stress in various tissues. It has been proved that activation of Unfolded Protein Response (UPR) under oxidative stress is a steady mechanism for maintaining cell function and survival. Therefore, the present study aimed to review the effect of Resistance Training (RT) and Berberine Chloride (BC) on the apoptosis-related UPR signaling pathway in the hippocampus of diazinon-poisoned rats.
Methods: In this experimental study, 40 male Wistar rats weighing 250 ±50 g were randomly divided into eight groups of five rats of 1) diazinon + 2 mg/kg BC + RT, 2) diazinon + 15 mg/kg BC + RT, 3) diazinon, 4) diazinon + RT, 5) diazinon + 2 mg/kg BC, 6) diazinon + 15 mg/ kg BC, 7) healthy control, and 8) sham. The groups were treated for 5 weeks. At the end of the fifth week, ATF-4, ATF-6, and CHOP gene expression in hippocampus tissue were measured by quantitative real-time RT-PCR.
Results: Diazinon significantly increased the expression of ATF-4, ATF-6, and CHOP in the hippocampus tissue of rats. Administrating 15 mg/kg BC with RT significantly decreased these genes, indicating a decrease in the rate of apoptosis in the hippocampus.
Conclusion: This study showed that RT and BC have a protective effect against diazinon-induced toxicity in the hippocampus.
Methods: In this experimental study, 40 male Wistar rats weighing 250 ±50 g were randomly divided into eight groups of five rats of 1) diazinon + 2 mg/kg BC + RT, 2) diazinon + 15 mg/kg BC + RT, 3) diazinon, 4) diazinon + RT, 5) diazinon + 2 mg/kg BC, 6) diazinon + 15 mg/ kg BC, 7) healthy control, and 8) sham. The groups were treated for 5 weeks. At the end of the fifth week, ATF-4, ATF-6, and CHOP gene expression in hippocampus tissue were measured by quantitative real-time RT-PCR.
Results: Diazinon significantly increased the expression of ATF-4, ATF-6, and CHOP in the hippocampus tissue of rats. Administrating 15 mg/kg BC with RT significantly decreased these genes, indicating a decrease in the rate of apoptosis in the hippocampus.
Conclusion: This study showed that RT and BC have a protective effect against diazinon-induced toxicity in the hippocampus.
Keywords: Berberine chloride, Diazinon, Resistance training, Activating Transcription Factor 4 (ATF-4), Activating Transcription Factor 6 (ATF-6), CHOP
Type of Study: Original |
Subject:
Behavioral Neuroscience
Received: 2019/12/4 | Accepted: 2020/03/5 | Published: 2021/05/1
Received: 2019/12/4 | Accepted: 2020/03/5 | Published: 2021/05/1
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