Introduction: Diazinon is one of the most widely used organophosphate pesticides in the world. This toxin enters the body in various ways and induces oxidative stress in various tissues. It has been proved that activation of UPR under oxidative stress is a consistent mechanism for maintaining cell function and survival. Therefore, present study aimed to review the effect of resistance training (RT) and berberine chloride (BC) on the apoptosis- related UPR signaling pathway in the hippocampus of diazinon- poisoned rats.
Methods: In this experimental study, 40 male Wistar rats weighing 250 ±50 g were randomly divided into eight groups of five rats including 1) diazinon + 2 mg/kg BC + RT, 2) diazinon + 15 mg/kg BC + RT, 3) diazinon, 4) diazinon + RT, 5) diazinon + 2 mg/kg BC, 6) diazinon + 15 mg/ kg BC, 7) healthy control, 8) sham. The groups were treated for 5 weeks. At the end of the fifth week, ATF-4, ATF-6 and CHOP gene expression in hippocampus tissue were measured by Quantitative Real Time RT-PCR.

Results: Diazinon significantly increased the expression of ATF-4, ATF-6, and CHOP in the hippocampus tissue of rats. 15 mg/kg BC with RT significantly decreased these gens, which may indicate a decrease in the rate of apoptosis in the hippocampus.

Conclusion: This study showed that RT along with BC has a protective effect against diazinon- induced toxicity in the hippocampus.

Keywords: Berberine Chloride, Diazinon, Resistance Training, ATF-4, ATF-6, CHOP

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