Volume 11, Issue 4 (July & August - Special Issue on Memory, Reward & Stress 2020)
BCN 2020, 11(4): 499-506 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Fahimi Truski F, Ghotbeddin Z, Tabandeh M R, Pourmahdi Borujeni M. Crocin Treatment after Maternal Hypoxia Attenuates Spatial Memory Impairment and Expression of BACE1 and HIF-1α in Rat Offspring Brain. BCN 2020; 11 (4) :499-506
URL: http://bcn.iums.ac.ir/article-1-1483-en.html
URL: http://bcn.iums.ac.ir/article-1-1483-en.html
1- Department of Physiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2- Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3- Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3- Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:
Objective: Hypoxia via expression of Hypoxia Inducible Factor-1 (HIF-1) is an important and effective factor in the onset and progression of memory disorders such as Alzheimer Disease (AD). The activity of β-secretase (BACE1) increased in hypoxia condition. BACE1 trigger a cascade of pathological events resulting in AD. Crocin act as memory improvement agent but its molecular mechanism is not well-known. So, in this study, the effect of crocin on spatial memory, HIF-1α and BACE1 gene expression were investigated in rat offspring under maternal hypoxia.
Materials and methods: Female pregnant rats on the 20th day of pregnancy were divided into 4 groups including: sham, crocin treated, hypoxia and hypoxia group treated with crocin. In hypoxia groups, pregnant rats were exposed to the 7% oxygen and 93% nitrogen intensity for 3 hours. In the crocin treated group, crocin (30 mg/kg) injected at P14-28, (i.p). At the end, Morris water maze was used to assess spatial memory and Real-Time PCR analysis was performed to measure the expression of BACE1 and HIF-1α genes in the brain of offspring.
Results: Maternal hypoxia impaired memory task compared to the sham group. But crocin treatment improved cognitive behavior. HIF-1α and BACE1 expression were unregulated in the brain of offspring in hypoxia group. Crocin treatment could attenuate the expression of both genes.
Conclusion: According to our results, down- regulation of HIF-1α and BACE1 gene expression in the brain of rat offspring after crocin treatment can be suggested as molecular mechanism for crocin to improve spatial memory.
Materials and methods: Female pregnant rats on the 20th day of pregnancy were divided into 4 groups including: sham, crocin treated, hypoxia and hypoxia group treated with crocin. In hypoxia groups, pregnant rats were exposed to the 7% oxygen and 93% nitrogen intensity for 3 hours. In the crocin treated group, crocin (30 mg/kg) injected at P14-28, (i.p). At the end, Morris water maze was used to assess spatial memory and Real-Time PCR analysis was performed to measure the expression of BACE1 and HIF-1α genes in the brain of offspring.
Results: Maternal hypoxia impaired memory task compared to the sham group. But crocin treatment improved cognitive behavior. HIF-1α and BACE1 expression were unregulated in the brain of offspring in hypoxia group. Crocin treatment could attenuate the expression of both genes.
Conclusion: According to our results, down- regulation of HIF-1α and BACE1 gene expression in the brain of rat offspring after crocin treatment can be suggested as molecular mechanism for crocin to improve spatial memory.
Type of Study: Original |
Subject:
Behavioral Neuroscience
Received: 2019/04/22 | Accepted: 2020/04/11 | Published: 2020/07/1
Received: 2019/04/22 | Accepted: 2020/04/11 | Published: 2020/07/1
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
