دوره 11، شماره 4 - ( 5-1399 )
جلد 11 شماره 4 صفحات 506-499 |
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Fahimi Truski F, Ghotbeddin Z, Tabandeh M R, Pourmahdi Borujeni M. Crocin Treatment after Maternal Hypoxia Attenuates Spatial Memory Impairment and Expression of BACE1 and HIF-1α in Rat Offspring Brain. BCN 2020; 11 (4) :499-506
URL: http://bcn.iums.ac.ir/article-1-1483-fa.html
URL: http://bcn.iums.ac.ir/article-1-1483-fa.html
Crocin Treatment after Maternal Hypoxia Attenuates Spatial Memory Impairment and Expression of BACE1 and HIF-1α in Rat Offspring Brain. مجله علوم اعصاب پایه و بالینی. 1399; 11 (4) :499-506
چکیده:
Objective: Hypoxia via expression of Hypoxia Inducible Factor-1 (HIF-1) is an important and effective factor in the onset and progression of memory disorders such as Alzheimer Disease (AD). The activity of β-secretase (BACE1) increased in hypoxia condition. BACE1 trigger a cascade of pathological events resulting in AD. Crocin act as memory improvement agent but its molecular mechanism is not well-known. So, in this study, the effect of crocin on spatial memory, HIF-1α and BACE1 gene expression were investigated in rat offspring under maternal hypoxia.
Materials and methods: Female pregnant rats on the 20th day of pregnancy were divided into 4 groups including: sham, crocin treated, hypoxia and hypoxia group treated with crocin. In hypoxia groups, pregnant rats were exposed to the 7% oxygen and 93% nitrogen intensity for 3 hours. In the crocin treated group, crocin (30 mg/kg) injected at P14-28, (i.p). At the end, Morris water maze was used to assess spatial memory and Real-Time PCR analysis was performed to measure the expression of BACE1 and HIF-1α genes in the brain of offspring.
Results: Maternal hypoxia impaired memory task compared to the sham group. But crocin treatment improved cognitive behavior. HIF-1α and BACE1 expression were unregulated in the brain of offspring in hypoxia group. Crocin treatment could attenuate the expression of both genes.
Conclusion: According to our results, down- regulation of HIF-1α and BACE1 gene expression in the brain of rat offspring after crocin treatment can be suggested as molecular mechanism for crocin to improve spatial memory.
Materials and methods: Female pregnant rats on the 20th day of pregnancy were divided into 4 groups including: sham, crocin treated, hypoxia and hypoxia group treated with crocin. In hypoxia groups, pregnant rats were exposed to the 7% oxygen and 93% nitrogen intensity for 3 hours. In the crocin treated group, crocin (30 mg/kg) injected at P14-28, (i.p). At the end, Morris water maze was used to assess spatial memory and Real-Time PCR analysis was performed to measure the expression of BACE1 and HIF-1α genes in the brain of offspring.
Results: Maternal hypoxia impaired memory task compared to the sham group. But crocin treatment improved cognitive behavior. HIF-1α and BACE1 expression were unregulated in the brain of offspring in hypoxia group. Crocin treatment could attenuate the expression of both genes.
Conclusion: According to our results, down- regulation of HIF-1α and BACE1 gene expression in the brain of rat offspring after crocin treatment can be suggested as molecular mechanism for crocin to improve spatial memory.
نوع مطالعه: Original |
موضوع مقاله:
Behavioral Neuroscience
دریافت: 1398/2/2 | پذیرش: 1399/1/23 | انتشار: 1399/4/11
دریافت: 1398/2/2 | پذیرش: 1399/1/23 | انتشار: 1399/4/11
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