Volume 11, Issue 1 (January & February 2020)
BCN 2020, 11(1): 49-58 |
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Namjoo E, Shekari M, Pirouzi A, Forouzandeh H, Khalafkhany D, Vahedi A et al . Haloperidol’s Effect on the Expressions of TGFB, NT-3, and BDNF genes in Cultured Rat Microglia. BCN 2020; 11 (1) :49-58
URL: http://bcn.iums.ac.ir/article-1-1087-en.html
URL: http://bcn.iums.ac.ir/article-1-1087-en.html
Elham Namjoo1 
, Mohammad Shekari2 , Aliyar Pirouzi3 , Hossein Forouzandeh * 3, Davod Khalafkhany4 , Abdolvahid Vahedi2 , Iraj Ahmadi5
, Mohammad Shekari2 , Aliyar Pirouzi3 , Hossein Forouzandeh * 3, Davod Khalafkhany4 , Abdolvahid Vahedi2 , Iraj Ahmadi5
1- Department of Biology, Faculty of Science, Arsenjan Branch, Islamic Azad University, Fars, Iran.
2- Genetics and Molecular Biology, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3- Gerash Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran
4- Molecular biology And Genetic Department, Bogazic University
5- Department of physiology, faculty of medicine, Ilam University of Medical Sciences, Ilam, Iran
2- Genetics and Molecular Biology, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3- Gerash Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran
4- Molecular biology And Genetic Department, Bogazic University
5- Department of physiology, faculty of medicine, Ilam University of Medical Sciences, Ilam, Iran
Abstract:
Introduction: Microglia, small glial cells, i.e. mesodermal in origin and found in the brain and spinal cord, play a key role in the maintenance of neurons and immune defense. Haloperidol, an antipsychotic drug, is used to treat numerous neurological and neurodegenerative disorders. Its mechanism is not understood; however, haloperidol may result in Wnt signaling pathway activation. This study aimed to activate the Wnt signaling pathway using haloperidol and determining the effect of GSK3 inhibition on the expression of TGFB, NT-3, and BDNF genes in cultured rat microglia.
Methods: Microglia isolation was conducted, and the immunohistochemistry technique was performed to confirm microglia purity. The RNA extraction was followed by cDNA synthesis. Real-time RT-PCR was used to evaluate any significant changes in the expression level of these genes.
Results: The three gene expressions in microglia were proportional to the different concentrations of the drug. More concentration of drugs resulted in higher levels of expression of these genes. Besides, the haloperidol did not affect the expression of the beta-actin gene as the reference gene.
Conclusion: The obtained results supported the beneficial use of haloperidol in targeted microglia therapy. This study can be a breakthrough in neurology research.
Methods: Microglia isolation was conducted, and the immunohistochemistry technique was performed to confirm microglia purity. The RNA extraction was followed by cDNA synthesis. Real-time RT-PCR was used to evaluate any significant changes in the expression level of these genes.
Results: The three gene expressions in microglia were proportional to the different concentrations of the drug. More concentration of drugs resulted in higher levels of expression of these genes. Besides, the haloperidol did not affect the expression of the beta-actin gene as the reference gene.
Conclusion: The obtained results supported the beneficial use of haloperidol in targeted microglia therapy. This study can be a breakthrough in neurology research.
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2017/12/1 | Accepted: 2019/04/29 | Published: 2020/01/1
Received: 2017/12/1 | Accepted: 2019/04/29 | Published: 2020/01/1
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