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1- Department of biology, faculty of Science, Islamic Azad University, Arsenjan branch, Fars, Iran
2- Genetics and Molecular Biology, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3- Gerash Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran
4- Department of physiology, faculty of medicine, Ilam University of Medical Sciences, Ilam, Iran
5- Molecular biology And Genetic Department, Bogazic University
Abstract:  
Microglia, small glia cells, which are mesodermal in origin and found in the brain and spinal cord, play a key role in maintenance of neurons and immune defense. Haloperidol, an antipsychotic drug, is used as a treatment for many neurological and neurodegenerative disorders while its mechanism is not totally understood but it is considered that haloperidol may result in WNT signaling pathway activation. The aim of this study was activating the WNT signaling pathway using haloperidol and determining the effect of GSK3 inhibition on the expression of TGFB, NT-3 and BDNF genes in cultured rat microglia. For this purpose, microglia isolation was done, and immunohistochemistry technique was performed to confirm microglia purity. RNA extraction was followed by cDNA synthesis. Real time RT-PCR was used to evaluate any significant changes in the expressions' level of these genes. These three genes' expressions in microglia were proportional to the different concentrations of drug.  More concentration of drug resulted in higher levels of expressions of these genes. It is notable that haloperidol had no effect on the expression of Beta actin gene as the reference gene. The result confirmed the benefit of using haloperidol in targeted microglia therapy. This study can be a breakthrough in neurology research.
 
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2017/12/1 | Accepted: 2019/04/29

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