Volume 10, Issue 5 (September & October 2019)                   BCN 2019, 10(5): 485-498 | Back to browse issues page

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Grüßer L, Blaumeiser-Debarry R, Rossaint R, Krings M, Kremer B, Höllig A et al . A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model. BCN 2019; 10 (5) :485-498
URL: http://bcn.iums.ac.ir/article-1-1019-en.html
1- Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
2- Department of Neurosurgery, RWTH Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany
3- Department of Neurosurgery, RWTH Aachen University Hospital, Aachen, Germany.
Introduction: Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a set-up. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied.
Methods: We defined necessary checkpoints and developed inclusion and exclusion criteria that take the observed variation in such a model into consideration. Objective measures include the identification and exclusion of pre-damaged slices and outliers. Six steps were outlined in this study.
Results: A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that through equal requirements set for all slices more precise findings are ensured.
Conclusion: Results in a TBI experiment on OHBS should be analyzed critically as inhomogeneities may occur. In order to ensure more precise findings, a structured approach of comparing the results should be followed. Further research is recommended to confirm and further develop this framework.
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Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2017/09/7 | Accepted: 2018/09/22 | Published: 2019/09/1

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