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1- Department of Anesthesiology, RWTH Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany
2- Department of Anesthesiology and Intensive Care, Medizinisches Zentrum StaedteRegion Aachen, Mauerfeldchen 25, 52146 Wuerselen, Germany
3- Department of Neurosurgery, RWTH Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany
Abstract:  
Introduction: Organotypic hippocampal brain slices (OHBS) provide an advantageous alternative to in-vivo models to scrutinize traumatic brain injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a set-up. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied.
Methods: We defined necessary checkpoints and developed inclusion and exclusion criteria that take the observed variation in such a model into consideration. Objective measures include the identification and exclusion of pre-damaged slices and outliers. Six steps were outlined.
Results: A six step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that through equal requirements set for all slices more precise findings are ensured.
Conclusion: Results in a TBI experiment on OHBS have to be analyzed critically as inhomogenities may occur. In order to ensure more precise findings, a structured approach of comparing the results should be followed. Further research is recommended to confirm and further develop this framework.
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2017/09/7 | Accepted: 2018/09/22

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