Volume 13, Issue 1 (January & February 2022)
BCN 2022, 13(1): 47-56 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Zeraatpisheh Z, Mirzaei E, Nami M, Alipour H, Ghasemian S, Azari H et al . A New and Simple Method for Spinal Cord Injury Induction in Mice. BCN 2022; 13 (1) :47-56
URL: http://bcn.iums.ac.ir/article-1-1642-en.html
URL: http://bcn.iums.ac.ir/article-1-1642-en.html
Zahra Zeraatpisheh1 
, Esmaeil Mirzaei2 , Mohammad Nami1 , Hamed Alipour3 , Somayeh Ghasemian4 , Hassan Azari5 , Hadi Aligholi * 1
, Esmaeil Mirzaei2 , Mohammad Nami1 , Hamed Alipour3 , Somayeh Ghasemian4 , Hassan Azari5 , Hadi Aligholi * 1
1- Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Department of Tissue Engineering & Applied cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Genetic Laboratory, Shiraz Fertility Center, Shiraz, Iran.
5- Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611, USA.
2- Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Department of Tissue Engineering & Applied cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Genetic Laboratory, Shiraz Fertility Center, Shiraz, Iran.
5- Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611, USA.
Abstract:
Introduction: Spinal Cord Injury (SCI) is a devastating disease with poor clinical outcomes. Animal models provide great opportunities to expand our horizons in identifying SCI pathophysiological mechanisms and introducing effective treatment strategies. The present study introduces a new murine contusion model.
Methods: A simple, cheap, and reproducible novel instrument was designed, which consisted of a body part, an immobilization piece, and a bar-shaped weight. The injury was inflicted to the spinal cord using an 8-g weight for 5, 10, or 15 minutes after laminectomy at the T9 level in male C57BL/6 mice. Motor function, cavity formation, cell injury, and macrophage infiltration were evaluated 28 days after injury.
Results: The newly designed instrument minimized adverse spinal movement during injury induction. Moreover, no additional devices, such as a stereotaxic apparatus, were required to stabilize the animals during the surgical procedure. Locomotor activity was deteriorated after injury. Furthermore, tissue damage and cell injury were exacerbated by increasing the duration of weight exertion. In addition, macrophage infiltration around the injured tissue was observed 28 days after injury.
Conclusion: This novel apparatus could induce a controllable SCI with a clear cavity formation in mice. No accessory elements are needed, which can be used in future SCI studies.
Methods: A simple, cheap, and reproducible novel instrument was designed, which consisted of a body part, an immobilization piece, and a bar-shaped weight. The injury was inflicted to the spinal cord using an 8-g weight for 5, 10, or 15 minutes after laminectomy at the T9 level in male C57BL/6 mice. Motor function, cavity formation, cell injury, and macrophage infiltration were evaluated 28 days after injury.
Results: The newly designed instrument minimized adverse spinal movement during injury induction. Moreover, no additional devices, such as a stereotaxic apparatus, were required to stabilize the animals during the surgical procedure. Locomotor activity was deteriorated after injury. Furthermore, tissue damage and cell injury were exacerbated by increasing the duration of weight exertion. In addition, macrophage infiltration around the injured tissue was observed 28 days after injury.
Conclusion: This novel apparatus could induce a controllable SCI with a clear cavity formation in mice. No accessory elements are needed, which can be used in future SCI studies.
Type of Study: Original |
Subject:
Behavioral Neuroscience
Received: 2019/10/28 | Accepted: 2020/07/12 | Published: 2022/01/1
Received: 2019/10/28 | Accepted: 2020/07/12 | Published: 2022/01/1
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
