Volume 6, Issue 4 (Automn 2015 -- 2015)
BCN 2015, 6(4): 253-264 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Yousef M A A, Dionigi P, Marconi S, Calligaro A, Cornaglia A I, Alfonsi E et al . Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental Device. BCN 2015; 6 (4) :253-264
URL: http://bcn.iums.ac.ir/article-1-637-en.html
URL: http://bcn.iums.ac.ir/article-1-637-en.html
Mohamed Abdelhamid Ali Yousef * 
1, Paolo Dionigi2 , Stefania Marconi2 , Alberto Calligaro2 , Antonia Icaro Cornaglia2 , Enrico Alfonsi2 , Ferdinando Auricchio2
1, Paolo Dionigi2 , Stefania Marconi2 , Alberto Calligaro2 , Antonia Icaro Cornaglia2 , Enrico Alfonsi2 , Ferdinando Auricchio2
1- Experimental Surgery and Microsurgery Research Unit, Department of General Surgery, University of Pavia, V.le Golgi 19, 27100, Pavia, Italy.
2- Organization
2- Organization
Abstract:
Introduction: Repair of peripheral nerve injuries is an intensive area of challenge and research in
modern reconstructive microsurgery. Intensive research is being carried out to develop effective
alternatives to the standard nerve autografting, avoiding its drawbacks. The aim of the study was
to evaluate the effectiveness of a newly designed mechanical device for the reconstruction of the
sciatic nerve in rats in comparison to nerve autografting and to assess the pain during the period
of distraction neurogenesis.
Methods: Fourteen Sprague Dawley rats were used and randomly assigned into 2 groups with
7 rats in each group group A (Nerve Autografting group) in which a 10-mm segment of the
sciatic nerve was resected and rotated 180 degrees, then primary end-to-end neurorrhaphy was
performed in the reverse direction group B (Nerve Lengthening group) in which the mechanical
device was inserted after surgical resection of 10 mm of the sciatic nerve, then secondary endto-
end neurorrhaphy was performed after completing the nerve lengthening. Thirteen weeks
later, assessment of the functional sciatic nerve recovery using static sciatic index (SSI) was
performed. Furthermore, fourteen weeks after the nerve resection, assessment of the nerve
regeneration with electrophysiological study and histological analysis were performed. Also,
gastrocnemius wet weight was measured. For pain assessment in group B, Rat Grimace Scale
(RGS) score was used.
Results: Significantly better functional recovery rate (using the SSI) was reported in the nerve
lengthening group in comparison to autografting group. Also, a statistically significant higher
nerve conduction velocity was detected in the nerve lengthening group. On histological analysis
of the distal nerve section at 3 mm distal to the nerve repair site, significant myelin sheath
thickness was detected in the nerve lengthening group.
Discussion: Distraction neurogenesis with the new experimental device is a reliable therapeutic
method for the reconstruction of nerve defects.
Type of Study: Original |
Subject:
Clinical Neuroscience
Received: 2015/02/21 | Accepted: 2015/06/14 | Published: 2015/10/1
Received: 2015/02/21 | Accepted: 2015/06/14 | Published: 2015/10/1
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
