Volume 6, Issue 1 (Winter 2015 -- 2015)
BCN 2015, 6(1): 52-57 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mostafavi H, Khaksarian M, Joghataei M T, Yoosefee S, Hadjighassem M R, Soleimannejad M, et al . cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells. BCN 2015; 6 (1) :52-57
URL: http://bcn.iums.ac.ir/article-1-493-en.html
URL: http://bcn.iums.ac.ir/article-1-493-en.html
Hossein Mostafavi1 
, Mojtaba Khaksarian2 , Mohammad Taghi Joghataei3 , Sadegh Yoosefee3 , Mahmoud Reza Hadjighassem * 4, Maryam Soleimannejad , Raheleh Gholamzadeh , Sanam Seifollahi Bahnamiri
, Mojtaba Khaksarian2 , Mohammad Taghi Joghataei3 , Sadegh Yoosefee3 , Mahmoud Reza Hadjighassem * 4, Maryam Soleimannejad , Raheleh Gholamzadeh , Sanam Seifollahi Bahnamiri
1- Department of Physiology and Pharmacology, Zanjan University of Medical Sciences
2- Department of Physiology, Lorestan University of Medical Sciences
3- Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences
4- Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences
2- Department of Physiology, Lorestan University of Medical Sciences
3- Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences
4- Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences
Abstract:
Introduction: Malignant astrocytic gliomas are the most common and lethal brain malignancies
due to their refractory to the current therapies. Nowadays, molecular targeted therapy has
attracted great attention in treatment of glioma. Connexin 43 (Cx43) and micro ribonucleic acid-
21(miR-21) are among molecules that are involved in glioma development and progression.
These molecules showed potential to be as target molecules with regard to glioma. Some studies
have reported that cyclic adenosine monophosphate (cAMP) signaling could be effective on
Cx43 and miR-21 in tissues other than in brain. We investigate possible relationship between
β-adrenergic receptor and its newly described downstream, exchange protein directly activated
by cAMP (Epac) signaling pathway and expression of Cx43 and miR-21 in low (1321N1) and
high grade (U87MG) glioma cell lines.
Methods: We treated cells with β-adrenergic agonist and Epac activator with and without adenyl
cyclase inhibitor. Cx43 and miR-21 expression were measured with real-time PCR.
Results: Our data showed that in 1321N1 cells, β-adrenergic-Epac pathway stimulation up and
down-regulated Cx43 and miR-21 expression respectively. Whereas, in U87MG cells these
interventions had no effect on Cx43 and miR-21 expression.
Discussion: These findings demonstrate that low grade astrocytoma cells have better response to
our pharmacological interventions.
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2014/05/23 | Accepted: 2014/10/11 | Published: 2015/01/1
Received: 2014/05/23 | Accepted: 2014/10/11 | Published: 2015/01/1
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
