Volume 5, Issue 2 (Spring 2014 2014)                   BCN 2014, 5(2): 117-123 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Havasi P, Nabioni M, Soleimani M, Bakhshandeh B, Morovvati H. The Proliferation Study of hiPS Cell-Derived Neuronal Progenitors on Poly-Caprolactone Scaffold. BCN 2014; 5 (2) :117-123
URL: http://bcn.iums.ac.ir/article-1-329-en.html
1- KHarazmi university
2- Kharazmi university
3- Tarbiat modars university
4- university of tehran
5- Shahid Chamran university
Abstract:  
Introduction: The native inability of nervous system to regenerate, encourage researchers to consider neural tissue engineering as a potential treatment for spinal cord injuries. Considering the suitable characteristics of induced pluripotent stem cells (iPSCs) for tissue regeneration applications, in this study we investigated the adhesion, viability and proliferation of neural progenitors (derived from human iPSCs) on aligned poly-caprolactone (PCL) nanofibers. Methods: Aligned poly-caprolactone nanofibrous scaffold was fabricated by electrospinning and characterized by scanning electron microscopy (SEM). Through neural induction, neural progenitor cells were derived from induced pluripotent stem cells. After cell seeding on the scaffolds, their proliferation was investigated on different days of culture. Results: According to the SEM micrographs, the electrospun PCL scaffolds were aligned along with uniformed morphology. Evaluation of adhesion and viability of neural progenitor cells on plate (control) and PCL scaffold illustrated increasing trends in proliferation but this rate was higher in scaffold group. The statistical analyses confirmed significant differences between groups on 36h and 48h. Discussion: Evaluation of cell proliferation along with morphological assessments, staining and SEM finding suggested biocompatibility of the PCL scaffolds and suitability of the combination of the mentioned scaffold and human iPS cells for neural regeneration.
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2013/01/10 | Accepted: 2013/07/31 | Published: 2014/04/1

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Basic and Clinical Neuroscience

Designed & Developed by : Yektaweb