Volume 16, Issue 6 (November & December- In Press 2025)
BCN 2025, 16(6): 0-0 |
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Haghani M, Namavar M R, Dehghani F, Naseh Z, Panahi Kokhdan S, Naseh M. Sodium Valproate Mitigates Hippocampal Structural and Functional Alterations Induced by Focal Cerebral Ischemia in Rats. BCN 2025; 16 (6)
URL: http://bcn.iums.ac.ir/article-1-3165-en.html
URL: http://bcn.iums.ac.ir/article-1-3165-en.html
Masoud Haghani1 
, Mohammad Reza Namavar1 , Farzaneh Dehghani1 , Zahra Naseh2 , Samira Panahi Kokhdan1 , Maryam Naseh *1
, Mohammad Reza Namavar1 , Farzaneh Dehghani1 , Zahra Naseh2 , Samira Panahi Kokhdan1 , Maryam Naseh *1
1- Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2- Center for Transfusion Medicine and Cellular Therapies, Department of Laboratory Medicine and Pathology, Emory University School of Medicine, Atlanta, GA
2- Center for Transfusion Medicine and Cellular Therapies, Department of Laboratory Medicine and Pathology, Emory University School of Medicine, Atlanta, GA
Abstract:
Purpose: Sodium Valproate (VPA) is widely used for the treatment of neurological diseases. The present study investigated the aspects of the spatial arrangement and three-dimensional structure of the CA1 region of the hippocampus, as well as the biochemical changes in the middle cerebral artery occlusion (MCAO) rats.
Methods: Thirty male Sprague-Dawley rats were randomly divided into three groups; Sham, MCAO, and MCAO+VPA. The right common carotid artery was occluded for one hour. VPA (300 mg/kg) or vehicle were injected intraperitoneally on days 0-3 of the reperfusion. On the 7th day after MCAO, the brains (ischemic hemisphere) of five animals were removed and fixed for structural (stereological) study. In addition, the ischemic hemispheres of five other rats were removed for malondialdehyde (MDA) assay.
Results: Our data showed that the administration of VPA could significantly recover the hippocampal spatial arrangement of the pyramidal neurons, and reduce three-dimensional deformation, and decrease the total number of dead neurons and MDA levels induced by MCAO.
Conclusions: The results of this study indicated that the reduction in neuronal death in the CA1 region of the hippocampus, along with the improvement in hippocampal spatial cell arrangement and three-dimensional structure, may be correlated with reduced levels of oxidative stress. These structural alterations may contribute to the behavioral effects previously reported, including in our earlier studies.
Methods: Thirty male Sprague-Dawley rats were randomly divided into three groups; Sham, MCAO, and MCAO+VPA. The right common carotid artery was occluded for one hour. VPA (300 mg/kg) or vehicle were injected intraperitoneally on days 0-3 of the reperfusion. On the 7th day after MCAO, the brains (ischemic hemisphere) of five animals were removed and fixed for structural (stereological) study. In addition, the ischemic hemispheres of five other rats were removed for malondialdehyde (MDA) assay.
Results: Our data showed that the administration of VPA could significantly recover the hippocampal spatial arrangement of the pyramidal neurons, and reduce three-dimensional deformation, and decrease the total number of dead neurons and MDA levels induced by MCAO.
Conclusions: The results of this study indicated that the reduction in neuronal death in the CA1 region of the hippocampus, along with the improvement in hippocampal spatial cell arrangement and three-dimensional structure, may be correlated with reduced levels of oxidative stress. These structural alterations may contribute to the behavioral effects previously reported, including in our earlier studies.
Keywords: Sodium Valproate, focal cerebral ischemia, hippocampal spatial arrangement, three-dimensional deformation, oxidative stress
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2025/03/11 | Accepted: 2025/08/31 | Published: 2025/11/7
Received: 2025/03/11 | Accepted: 2025/08/31 | Published: 2025/11/7
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