Volume 16, Issue 6 (November & December 2025)
BCN 2025, 16(6): 1159-1168 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Haghani M, Namavar M R, Dehghani F, Vatanparast J, Naseh Z, Kokhdan S P et al . Sodium Valproate Mitigates Hippocampal Structural and Functional Alterations Following Middle Cerebral Artery Occlusion in a Rat Model. BCN 2025; 16 (6) :1159-1168
URL: http://bcn.iums.ac.ir/article-1-3165-en.html
URL: http://bcn.iums.ac.ir/article-1-3165-en.html
Masoud Haghani1 
, Mohammad Reza Namavar2 , Farzaneh Dehghani3 , Jafar Vatanparast4 , Zahra Naseh5 , Samira Panahi Kokhdan6 , Maryam Naseh *7
, Mohammad Reza Namavar2 , Farzaneh Dehghani3 , Jafar Vatanparast4 , Zahra Naseh5 , Samira Panahi Kokhdan6 , Maryam Naseh *7
1- Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. & Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. & Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. & Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. & Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran.
5- Department of Laboratory Medicine and Pathology, Center for Transfusion Medicine and Cellular Therapies, School of Medicine, Emory University, Atlanta, United States.
6- Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
7- Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. & Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. & Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. & Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran.
5- Department of Laboratory Medicine and Pathology, Center for Transfusion Medicine and Cellular Therapies, School of Medicine, Emory University, Atlanta, United States.
6- Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
7- Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract:
Introduction: Sodium valproate (VPA) is widely used to treat neurological disorders. This study aimed to investigate the spatial arrangement and three-dimensional structure of the hippocampal CA1 region, as well as the biochemical changes in the brains of ischemic rats.
Methods: Thirty male rats were randomly assigned to three groups: Sham, middle cerebral artery occlusion (MCAO), and MCAO + VPA. The right common carotid artery was ligated for one h. On days 0–3 of reperfusion, animals received intraperitoneal (IP) injections of VPA (300 mg/kg). One week after MCAO, the brains (ischemic hemisphere) of five animals were removed and fixed for structural (stereological) studies. In addition, the ischemic hemispheres of five other rats were removed for malondialdehyde (MDA) assay.
Results: Our data demonstrated that VPA significantly restored the hippocampal spatial arrangement of pyramidal neurons, reduced three-dimensional deformation, and decreased the total number of dead neurons and MDA levels induced by MCAO.
Conclusion: This study indicates that reduced neuronal loss in the CA1 region, along with improvements in spatial cell arrangement and three-dimensional structure, may be correlated with reduced levels of oxidative stress. These structural alterations may contribute to the behavioral effects previously reported, including those in our earlier studies.
Methods: Thirty male rats were randomly assigned to three groups: Sham, middle cerebral artery occlusion (MCAO), and MCAO + VPA. The right common carotid artery was ligated for one h. On days 0–3 of reperfusion, animals received intraperitoneal (IP) injections of VPA (300 mg/kg). One week after MCAO, the brains (ischemic hemisphere) of five animals were removed and fixed for structural (stereological) studies. In addition, the ischemic hemispheres of five other rats were removed for malondialdehyde (MDA) assay.
Results: Our data demonstrated that VPA significantly restored the hippocampal spatial arrangement of pyramidal neurons, reduced three-dimensional deformation, and decreased the total number of dead neurons and MDA levels induced by MCAO.
Conclusion: This study indicates that reduced neuronal loss in the CA1 region, along with improvements in spatial cell arrangement and three-dimensional structure, may be correlated with reduced levels of oxidative stress. These structural alterations may contribute to the behavioral effects previously reported, including those in our earlier studies.
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2025/03/11 | Accepted: 2025/08/31 | Published: 2025/11/7
Received: 2025/03/11 | Accepted: 2025/08/31 | Published: 2025/11/7
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
