Volume 16, Issue 4 (July & August 2025)
BCN 2025, 16(4): 751-762 |
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Momeni J, Hosseini E, Khaleghi Ghadiri M, Samini F, Tabibkhooei A, Gorji A et al . The Impact of Small Molecule on the Astrocyte's Viability Derived From Epileptic Brain Tissues. BCN 2025; 16 (4) :751-762
URL: http://bcn.iums.ac.ir/article-1-3098-en.html
URL: http://bcn.iums.ac.ir/article-1-3098-en.html
Javad Momeni1 
, Elham Hosseini1 , Maryam Khaleghi Ghadiri2 , Fariborz Samini3 , Alireza Tabibkhooei4 , Ali Gorji5 , Sajad Sahab Negah *6
, Elham Hosseini1 , Maryam Khaleghi Ghadiri2 , Fariborz Samini3 , Alireza Tabibkhooei4 , Ali Gorji5 , Sajad Sahab Negah *6
1- Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. & Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
2- Department of Neurosurgery, Münster University, Münster, Germany.
3- Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
4- Department of Neurosurgery, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
5- Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
6- Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Multiple Sclerosis Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.
2- Department of Neurosurgery, Münster University, Münster, Germany.
3- Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
4- Department of Neurosurgery, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
5- Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
6- Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Multiple Sclerosis Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.
Abstract:
Introduction: Astrocyte dysfunction plays a crucial role in epileptogenesis by impacting neuronal excitability and synaptic transmission. This study investigates how specific small molecules (SMs) affect the survival of astrocytes derived from various brain regions (the neocortex, hippocampus, or amygdala) of patients with medically refractory epilepsy.
Methods: This study evaluated astrocyte responses to three distinct SMs (valproate, forskolin, and GSK3 inhibitor/WNT activator CHIR99021), individually or in combined forms, to determine their differential effects on astrocyte survival.
Results: The effects of SMs on astrocyte survival varied based on the brain tissue source and individual patient differences. Astrocytes from the amygdala of two patients showed heightened sensitivity to the SMs, while astrocytes from the neocortex of one patient exhibited decreased viability following treatment with CHIR99021 and valproate. Moreover, astrocytes from the hippocampus exhibited a significant decrease in viability in one patient, whereas no significant changes were observed in other patients.
Conclusion: Variability in astrocyte responses to SMs, influenced by brain region and patient differences, may highlight their role in shaping diverse therapeutic outcomes in individuals. Further studies are required to clarify the factors involved in the different behaviors of astrocytes in response to SMs in epilepsy.
Methods: This study evaluated astrocyte responses to three distinct SMs (valproate, forskolin, and GSK3 inhibitor/WNT activator CHIR99021), individually or in combined forms, to determine their differential effects on astrocyte survival.
Results: The effects of SMs on astrocyte survival varied based on the brain tissue source and individual patient differences. Astrocytes from the amygdala of two patients showed heightened sensitivity to the SMs, while astrocytes from the neocortex of one patient exhibited decreased viability following treatment with CHIR99021 and valproate. Moreover, astrocytes from the hippocampus exhibited a significant decrease in viability in one patient, whereas no significant changes were observed in other patients.
Conclusion: Variability in astrocyte responses to SMs, influenced by brain region and patient differences, may highlight their role in shaping diverse therapeutic outcomes in individuals. Further studies are required to clarify the factors involved in the different behaviors of astrocytes in response to SMs in epilepsy.
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2024/12/14 | Accepted: 2025/01/22 | Published: 2025/07/1
Received: 2024/12/14 | Accepted: 2025/01/22 | Published: 2025/07/1
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