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Gelareh Vahabzade1 
, Ashrafsadat Moazam1 , Mandana Rahimi2 , Fatemeh Khajehasani3 , Manijeh Motevalian *4
, Ashrafsadat Moazam1 , Mandana Rahimi2 , Fatemeh Khajehasani3 , Manijeh Motevalian *4
1- Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. 2. Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
2- Hasheminejad Kidney Center, Pathology Department, Iran University of Medical Sciences, Tehran, Iran.
3- Radiology Department, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
4- Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. & Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
2- Hasheminejad Kidney Center, Pathology Department, Iran University of Medical Sciences, Tehran, Iran.
3- Radiology Department, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
4- Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. & Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
Abstract:
Introduction: Reactive gliosis contributes to damage and recovery patterns in hydrocephalus. The purpose of the research was to evaluate the impact of Origanum vulgare essential oil (OEO) on reactive astrogliosis and decreased lateral ventricle thickness in a rat model of kaolin-induced hydrocephalus.
Methods: 30 male Wistar rats were injected with 25% kaolin directly into the cisterna magna. After 21 days post-injection of kaolin, hydrocephalic rats were randomly divided into four groups, and the presence of hydrocephalus was confirmed by magnetic resonance imaging (MRI) to determine the lateral ventricle volume. Rats received different doses of OEO for 10 days. Also, to assess the expression levels of Iba-1 and GFAP proteins, immunohistochemical and western blot analyses were performed 10 days after the treatment course.
Results: The size of the lateral ventricle in hydrocephalic rats treated with OEO was less than in those treated with kaolin alone. Based on GFAP and Iba-1 immunostaining data, hydrocephalic rats showed more reactive astrocytes and activated microglia than OEO-treated animals. The findings of the immunohistochemistry analysis were also validated by western blot. In terms of GFAP and Iba-1 expression levels, there was a significant difference between the hydrocephalic control group and rats treated with OEO at a dose of 200 mg/kg/day.
Conclusion: In comparison to hydrocephalic rats, the OEO-treated groups exhibited stable ventricular dilatation. According to the results of this research, OEO may reduce hydrocephalus-related damage in kaolin-induced rat brains, potentially through lowering GFAP and Iba-1, and so OEO intervention might be effective as a novel treatment technique.
Methods: 30 male Wistar rats were injected with 25% kaolin directly into the cisterna magna. After 21 days post-injection of kaolin, hydrocephalic rats were randomly divided into four groups, and the presence of hydrocephalus was confirmed by magnetic resonance imaging (MRI) to determine the lateral ventricle volume. Rats received different doses of OEO for 10 days. Also, to assess the expression levels of Iba-1 and GFAP proteins, immunohistochemical and western blot analyses were performed 10 days after the treatment course.
Results: The size of the lateral ventricle in hydrocephalic rats treated with OEO was less than in those treated with kaolin alone. Based on GFAP and Iba-1 immunostaining data, hydrocephalic rats showed more reactive astrocytes and activated microglia than OEO-treated animals. The findings of the immunohistochemistry analysis were also validated by western blot. In terms of GFAP and Iba-1 expression levels, there was a significant difference between the hydrocephalic control group and rats treated with OEO at a dose of 200 mg/kg/day.
Conclusion: In comparison to hydrocephalic rats, the OEO-treated groups exhibited stable ventricular dilatation. According to the results of this research, OEO may reduce hydrocephalus-related damage in kaolin-induced rat brains, potentially through lowering GFAP and Iba-1, and so OEO intervention might be effective as a novel treatment technique.
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2024/07/16 | Accepted: 2025/06/10
Received: 2024/07/16 | Accepted: 2025/06/10
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