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1- Physiology Research Center, Department of Physiology, Iran University of Medical Sciences, Tehran, Iran.
2- School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
2- School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Abstract:
Purpose:While Genotropin, a recombinant human growth hormone (GH), may be a repositioning drug for treating neurological diseases, its effectiveness for neuropathic pain remains uncertain. The current research investigated the pain alleviating effect of Genotropin and its possible effective mechanisms.
Method: Two weeks after chronic constriction injury (CCI) of sciatic nerve, adult male rats were divided into three main experimental groups: Control, Vehicle which received normal saline, and Treatment which received Genotropin (0.3 and 0.6 mg/kg) either alone or in combination with L-arginine, L-NAME, or glutamate (n=8). Pain-related behaviors were assessed using Von Frey filaments, plantar and the Randall-Selitto tests. Blood samples were collected to evaluate relevant oxidant/antioxidant markers.
Results: GH decreased mechanical allodynia (P<0.05, F=2.7) mechanical (P<0.01, F=3.4) and thermal hyperalgesia (P<0.001, F=2.5). Also, pretreatment with 0.3 mg/kg GH abolished the nociceptive effects of L-arginine (500mg/kg) and glutamate (1000nmol) (P<0.01; F=2, F=3), while enhancing the antinociceptive effect of L-NAME (P< 0.05, F=2.8). It significantly reduced lipid peroxidation (P < 0.01, F =3.7), restored glutathione, glutathione peroxidase, and superoxide dismutase levels (P < 0.01, F =11, F = 10.52, F = 5 respectively) and increased the catalase level (P < 0.01, F =5) in plasma.
Conclusion: The current data suggests that exogenous GH, alleviates pain and enhance antioxidative factors in the peripheral neuropathic pain model. The glutamate and nitric oxide pathways are also involved in its’ antinociceptive effect. It seems that Genotropin can be effective as a repositioning drug in the treatment of neuropathic pain.
Method: Two weeks after chronic constriction injury (CCI) of sciatic nerve, adult male rats were divided into three main experimental groups: Control, Vehicle which received normal saline, and Treatment which received Genotropin (0.3 and 0.6 mg/kg) either alone or in combination with L-arginine, L-NAME, or glutamate (n=8). Pain-related behaviors were assessed using Von Frey filaments, plantar and the Randall-Selitto tests. Blood samples were collected to evaluate relevant oxidant/antioxidant markers.
Results: GH decreased mechanical allodynia (P<0.05, F=2.7) mechanical (P<0.01, F=3.4) and thermal hyperalgesia (P<0.001, F=2.5). Also, pretreatment with 0.3 mg/kg GH abolished the nociceptive effects of L-arginine (500mg/kg) and glutamate (1000nmol) (P<0.01; F=2, F=3), while enhancing the antinociceptive effect of L-NAME (P< 0.05, F=2.8). It significantly reduced lipid peroxidation (P < 0.01, F =3.7), restored glutathione, glutathione peroxidase, and superoxide dismutase levels (P < 0.01, F =11, F = 10.52, F = 5 respectively) and increased the catalase level (P < 0.01, F =5) in plasma.
Conclusion: The current data suggests that exogenous GH, alleviates pain and enhance antioxidative factors in the peripheral neuropathic pain model. The glutamate and nitric oxide pathways are also involved in its’ antinociceptive effect. It seems that Genotropin can be effective as a repositioning drug in the treatment of neuropathic pain.
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2024/04/6 | Accepted: 2024/12/4
Received: 2024/04/6 | Accepted: 2024/12/4
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