Volume 14, Issue 1 (January & February 2023)
BCN 2023, 14(1): 31-42 |
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Entezari M, Bakhtiari M, Moradi F, Mozafari M, Bagher Z, Soleimani M. Human Olfactory Ecto-mesenchymal Stem Cells Displaying Schwann-cell-like Phenotypes and Promoting Neurite Outgrowth in Vitro. BCN 2023; 14 (1) :31-42
URL: http://bcn.iums.ac.ir/article-1-2210-en.html
URL: http://bcn.iums.ac.ir/article-1-2210-en.html
Maedeh Entezari1 
, Mehrdad Bakhtiari1 , Fatemeh Moradi1 , Masoud Mozafari2 , Zohreh Bagher3 , Mansoureh Soleimani * 1
, Mehrdad Bakhtiari1 , Fatemeh Moradi1 , Masoud Mozafari2 , Zohreh Bagher3 , Mansoureh Soleimani * 1
1- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. ; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. ; ENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. ; ENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract:
Introduction: Strategies of Schwann cell (SC) transplantation for regeneration of peripheral nerve injury involve many limitations. Stem cells can be used as alternative cell source for differentiation into Schwann cells. Given the high potential of neural crest-derived stem cells for the generation of multiple cell lineages, in this research, we considered whether olfactory ectomesenchymal stem cells (OE-MSCs) derived from neural crest can spontaneously differentiate into SC lineage
Methods: OE-MSCs were isolated from human nasal mucosa and characterized by the mesenchymal and neural crest markers. The cells were cultured in glial growth factors-free medium and further investigated in terms of the phenotypic and functional properties.
Results: Immunocytochemical staining and real-time PCR analysis indicated that the cultured OE-MSCs expressed SCs markers, SOX10, p75, S100, GFAP and MBP, differentiation indicative. It was found that the cells could secrete neurotrophic factors, including brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Furthermore, after co-cultured with PC12, the mean neurite length was enhanced by OE-MSCs.
Conclusion: The findings indicated that OE-MSCs could be differentiated spontaneously into SC-like phenotypes, suggesting their applications for transplantation in peripheral nerve injuries.
Methods: OE-MSCs were isolated from human nasal mucosa and characterized by the mesenchymal and neural crest markers. The cells were cultured in glial growth factors-free medium and further investigated in terms of the phenotypic and functional properties.
Results: Immunocytochemical staining and real-time PCR analysis indicated that the cultured OE-MSCs expressed SCs markers, SOX10, p75, S100, GFAP and MBP, differentiation indicative. It was found that the cells could secrete neurotrophic factors, including brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Furthermore, after co-cultured with PC12, the mean neurite length was enhanced by OE-MSCs.
Conclusion: The findings indicated that OE-MSCs could be differentiated spontaneously into SC-like phenotypes, suggesting their applications for transplantation in peripheral nerve injuries.
Keywords: Spontaneous differentiation, Olfactory ecto-mesenchymal stem cells, Schwann cell, Peripheral nerve regeneration
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2021/07/4 | Accepted: 2021/09/11 | Published: 2023/01/1
Received: 2021/07/4 | Accepted: 2021/09/11 | Published: 2023/01/1
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