Volume 15, Issue 2 (March & April 2024)                   BCN 2024, 15(2): 221-232 | Back to browse issues page

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Hansen E, Janson C, Romanova L, Lam C. Effect of Parenchymal Arachnoid on Brain Fluid Transport. BCN 2024; 15 (2) :221-232
URL: http://bcn.iums.ac.ir/article-1-2011-en.html
1- Department of Neurosurgery, Minneapolis Veterans Administration Health Care System, Minneapolis, United States.
2- Department of Internal Medicine and Neurology, Wright State University, Beavercreek, United States.
3- Department of Neurology and Rehabilitation, Medical School, University of Illinois Chicago, Chicago, United States.
4- Department of Neurosurgery, School of Medicine, University of Minnesota, Minneapolis, United States.

Introduction: The pia-arachnoid is a critical component of cerebrospinal fluid removal. It covers and invaginates into the brain parenchyma, and physiologic failure results in hydrocephalus and cerebral edema. The purpose of this study was to characterize the role of arachnoid within brain parenchyma and determine if water flux and solute transport are affected by these intra-parenchymal cells.
Methods: An immortalized arachnoid rat cell line was used to seed 300-µm organotypic rat brain slices of 4-week-old rats. Fluid and tracer transport analyses were conducted following a 7-10 day intraparenchymal growth period. The development of an arachnoid brain slice model was characterized using diffusion chamber experiments to calculate permeability, diffusion coefficient, and flux. 
Results: Labeled rat arachnoid cells readily penetrated organotypic cultures for up to 10 days. A significant reduction of dye and water flux across arachnoid-impregnated brain slices was observed after 3 hours in the diffusion chamber. Permeability decreased in whole brain slices containing arachnoid cells compared to slices without arachnoid cells. In comparison, a significant reduction of dextran across all slices occurred when molecular weights increased from 40 to 70 kDa. 
Conclusion: Tracer and small molecule studies show that arachnoid cells’ presence significantly impacts water’s movement through brain parenchyma. Size differential experiments indicate that the permeability of solute changed substantially between 40 and 70 kDa, an essential marker of blood-CSF barrier definition. We have developed an arachnoid organotypic model that reveals their ability to alter permeability and transport.

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Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2020/11/30 | Accepted: 2022/06/27 | Published: 2024/03/1

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