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1- Assistant Professor Tabriz, University of Tabriz, Faculty of Veterinary Medicine, Department of Basic Science, Division of Pharmacology and Toxicology - Faculty of Veterinary Medicine, University of Tabriz, Shohadaye Ghavvas Blvd, Opposite to Khavaran Town, Tabriz, East Azarbaijan Province, Iran.
2- Student Tabriz, University of Tabriz, Faculty of Veterinary Medicine, Department of Basic Science, Division of Pharmacology and Toxicology - Faculty of Veterinary Medicine, University of Tabriz, Shohadaye Ghavvas Blvd, Opposite to Khavaran Town, Tabriz, East Azarbaijan Province, Iran.
3- Assistant Professor Tabriz, University of Tabriz, Faculty of Veterinary Medicine, Department of Basic Science, Division of Physiology - Faculty of Veterinary Medicine, University of Tabriz, Shohadaye Ghavvas Blvd, Opposite to Khavaran Town, Tabriz, East Azarbaijan Province, Iran.
2- Student Tabriz, University of Tabriz, Faculty of Veterinary Medicine, Department of Basic Science, Division of Pharmacology and Toxicology - Faculty of Veterinary Medicine, University of Tabriz, Shohadaye Ghavvas Blvd, Opposite to Khavaran Town, Tabriz, East Azarbaijan Province, Iran.
3- Assistant Professor Tabriz, University of Tabriz, Faculty of Veterinary Medicine, Department of Basic Science, Division of Physiology - Faculty of Veterinary Medicine, University of Tabriz, Shohadaye Ghavvas Blvd, Opposite to Khavaran Town, Tabriz, East Azarbaijan Province, Iran.
Abstract:
Introduction: The present research aims to investigate the protective action of menthol dissolved in dimethyl sulfoxide (DMSO) on experimental epileptiform activity induced by the intraperitoneal (i.p.) injection of pentylenetetrazol (PTZ) in male rats.
Methods: Thirty adult male Wistar rats, weighing 200-250 g were randomly assigned to five equal groups. The control animals received normal saline (200 µl) and the rest four cohorts were considered as treatments. Menthol was dissolved in DMSO and intraperitoneally injected at the doses of 100, 200, and 400 mg/kg into the first, second, and third groups (M100, M200, and M400 V=200 µl), respectively. The fourth treatment was injected with the solvent (200 µl). The animals were anesthetized, then underwent cranial surgery and a recording electrode was implanted in the stratum radiatum of the hippocampal CA1 region (AP: -2.76 mm, ML: -1.4 mm and DV: 3 mm). The seizure activity was induced by PTZ (i.p.) and assessed through counting and measuring amplitudes of the spikes for 10 minutes using the eTrace program.
Results: It was noticed that menthol significantly reduced the activity level of PTZ-induced epileptiform activity, as well as dose-dependently exerted protective and inhibitory action on proconvulsant effect of DMSO.
Conclusion: Menthol can potentially be used as an adjuvant to prevent seizure activity.
Methods: Thirty adult male Wistar rats, weighing 200-250 g were randomly assigned to five equal groups. The control animals received normal saline (200 µl) and the rest four cohorts were considered as treatments. Menthol was dissolved in DMSO and intraperitoneally injected at the doses of 100, 200, and 400 mg/kg into the first, second, and third groups (M100, M200, and M400 V=200 µl), respectively. The fourth treatment was injected with the solvent (200 µl). The animals were anesthetized, then underwent cranial surgery and a recording electrode was implanted in the stratum radiatum of the hippocampal CA1 region (AP: -2.76 mm, ML: -1.4 mm and DV: 3 mm). The seizure activity was induced by PTZ (i.p.) and assessed through counting and measuring amplitudes of the spikes for 10 minutes using the eTrace program.
Results: It was noticed that menthol significantly reduced the activity level of PTZ-induced epileptiform activity, as well as dose-dependently exerted protective and inhibitory action on proconvulsant effect of DMSO.
Conclusion: Menthol can potentially be used as an adjuvant to prevent seizure activity.
Type of Study: Original |
Subject:
Clinical Neuroscience
Received: 2020/11/14 | Accepted: 2022/05/30
Received: 2020/11/14 | Accepted: 2022/05/30
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