Volume 14, Issue 4 (July & August 2023)
BCN 2023, 14(4): 453-462 |
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Hashemi L, Soodi M, Hajimehdipoor H, Dashti A. Ferulago angulata Methanolic Extract Protects PC12 Cells Against Beta-amyloid-induced Toxicity. BCN 2023; 14 (4) :453-462
URL: http://bcn.iums.ac.ir/article-1-1979-en.html
URL: http://bcn.iums.ac.ir/article-1-1979-en.html
1- Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
2- Institute for Natural Products and Medicinal Plants, Tarbiat Modares University, Tehran, Iran.
3- Department of Traditional Pharmacy, Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Forensic Toxicology, Legal Medicine Research Center, Iranian Legal Medicine Organization, Tehran, Iran.
2- Institute for Natural Products and Medicinal Plants, Tarbiat Modares University, Tehran, Iran.
3- Department of Traditional Pharmacy, Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Forensic Toxicology, Legal Medicine Research Center, Iranian Legal Medicine Organization, Tehran, Iran.
Abstract:
Introduction: Alzheimer’s disease (AD) is an age-dependent neurodegenerative disease. Beta-amyloid (Aβ)-induced neurotoxicity has a pivotal role in AD pathogenesis; therefore, the modulation of Aβ toxicity is the promising therapeutic approach to control the disease progression. Medicinal plants because of their multiple active ingredients are effective in complex diseases, such as AD. Therefore, several studies have studied medicinal plants to find an effective treatment for AD. Ferulago angulata is a medicinal plant with antioxidant and neuroprotective activity. The present study was done to assess the protective effect of the methanolic extract of Ferulago angulate on Aβ-induced toxicity and oxidative stress in PC12 cells.
Methods: The methanolic extract of aerial parts of the plant was prepared by the maceration method. PC12 cells were cultured according to a standard protocol. PC12 cells were incubated for 24 hours with Aβ alone, and Aβ in combination with various concentrations of the F. angulata extract. Cell viability was determined by the methyl thiazole tetrazolium (MTT) assay. Also, reactive oxygen species (ROS) production and the activity of acetylcholine esterase (AChE), glutathione peroxidase (GPx), and caspase-3 enzymes were measured.
Results:The extract dose-dependently protected PC12 cells against Aβ-induced cell death. Also, Aβ increased ROS production, AChE, and caspase-3 activity, and decreased the GPx activity, which all were ameliorated by F. angulata extract.
Conclusion: F. angulata extract protects against Aβ-induced oxidative stress and apoptosis. These effects may be due to the antioxidant and anticholinesterase activity of the extract. It is recommended to assess F. angulata extract as an anti-AD agent.
Methods: The methanolic extract of aerial parts of the plant was prepared by the maceration method. PC12 cells were cultured according to a standard protocol. PC12 cells were incubated for 24 hours with Aβ alone, and Aβ in combination with various concentrations of the F. angulata extract. Cell viability was determined by the methyl thiazole tetrazolium (MTT) assay. Also, reactive oxygen species (ROS) production and the activity of acetylcholine esterase (AChE), glutathione peroxidase (GPx), and caspase-3 enzymes were measured.
Results:The extract dose-dependently protected PC12 cells against Aβ-induced cell death. Also, Aβ increased ROS production, AChE, and caspase-3 activity, and decreased the GPx activity, which all were ameliorated by F. angulata extract.
Conclusion: F. angulata extract protects against Aβ-induced oxidative stress and apoptosis. These effects may be due to the antioxidant and anticholinesterase activity of the extract. It is recommended to assess F. angulata extract as an anti-AD agent.
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2020/08/26 | Accepted: 2021/03/6 | Published: 2023/07/1
Received: 2020/08/26 | Accepted: 2021/03/6 | Published: 2023/07/1
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