Volume 13, Issue 5 (September & October 2022)
BCN 2022, 13(5): 695-708 |
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Igbo E J, Okoliko U, Aminu I, Kopada A, Olorunnado S, Akinola O B. Structural Changes in the Medial Prefrontal Cortex and Anterior Cingulate Cortex of Dehydroepiandrosterone-Induced Wistar Rat Model of Polycystic Ovarian Syndrome. BCN 2022; 13 (5) :695-708
URL: http://bcn.iums.ac.ir/article-1-1975-en.html
URL: http://bcn.iums.ac.ir/article-1-1975-en.html
Enya Joseph Igbo * 
1, Ukwenya Okoliko2 , Imam Aminu1 , Aisha Kopada1 , Samson Olorunnado3 , Oluwole B. Akinola1
1, Ukwenya Okoliko2 , Imam Aminu1 , Aisha Kopada1 , Samson Olorunnado3 , Oluwole B. Akinola1
1- Department of Anatomy, University of Ilorin, Ilorin, Kwara State, Nigeria.
2- Department of Human Anatomy, Federal University of Technology, Akure, Ondo, Nigeria.
3- Department of Human Anatomy, School of Medicine and Pharmacy, College of Medicine and Health Sciences, University of Rwanda, Kigali, Rwanda.
2- Department of Human Anatomy, Federal University of Technology, Akure, Ondo, Nigeria.
3- Department of Human Anatomy, School of Medicine and Pharmacy, College of Medicine and Health Sciences, University of Rwanda, Kigali, Rwanda.
Abstract:
Introduction: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder in women that is associated with an increased risk of infertility. This study aims to evaluate the neurobehavioral and neurochemical changes along with the associated changes in the medial prefrontal cortex (mPFC) and anterior cingulate cortex (ACC) of the dehydroepiandrosterone (DHEA)-induced PCOS model rats.
Methods: A total of 12 female juvenile Wistar rats (30 to 50 g) about 22 to 44 days old were divided into 2 groups. The control group received sesame oil while the PCOS group received sesame oil plus DHEA. All treatment was done via daily subcutaneous injection for 21 days.
Results: Subcutaneous DHEA-induced PCOS significantly depleted the line crossing and rearing frequency in the open field, along with the percentage of the time in the white box, line crossing, rearing, and peeping frequency in the black and white box, and the percentage of alternation in the Y-maze. PCOS significantly increased the immobility time, freezing period, and the percentage of time in the dark area in the forced swim test, open field test, and black and white box, respectively. The level of luteinizing hormone, follicle-stimulating hormone, malondialdehyde (MDA), reactive oxygen species (ROS), and interleukin-6 (IL-6) increased significantly, while norepinephrine depleted significantly with an obvious decrease in the brain-derived neurotrophic factor level in the PCOS model rats. PCOS rats exhibited cystic follicles in the ovaries and necrotic or degenerative like features in the hippocampal pyramidal cells.
Conclusion: DHEA-induced PCOS results in anxiety and depressive behavior with structural alteration in rats, possibly through the elevation of MDA, ROS, and IL-6 levels, which also attributes to impaired emotional and executive functions in the mPFC and ACC.
Methods: A total of 12 female juvenile Wistar rats (30 to 50 g) about 22 to 44 days old were divided into 2 groups. The control group received sesame oil while the PCOS group received sesame oil plus DHEA. All treatment was done via daily subcutaneous injection for 21 days.
Results: Subcutaneous DHEA-induced PCOS significantly depleted the line crossing and rearing frequency in the open field, along with the percentage of the time in the white box, line crossing, rearing, and peeping frequency in the black and white box, and the percentage of alternation in the Y-maze. PCOS significantly increased the immobility time, freezing period, and the percentage of time in the dark area in the forced swim test, open field test, and black and white box, respectively. The level of luteinizing hormone, follicle-stimulating hormone, malondialdehyde (MDA), reactive oxygen species (ROS), and interleukin-6 (IL-6) increased significantly, while norepinephrine depleted significantly with an obvious decrease in the brain-derived neurotrophic factor level in the PCOS model rats. PCOS rats exhibited cystic follicles in the ovaries and necrotic or degenerative like features in the hippocampal pyramidal cells.
Conclusion: DHEA-induced PCOS results in anxiety and depressive behavior with structural alteration in rats, possibly through the elevation of MDA, ROS, and IL-6 levels, which also attributes to impaired emotional and executive functions in the mPFC and ACC.
Keywords: Polycystic ovary syndrome, Medial Prefrontal Cortex (mPFC), Anterior Cingulate Cortex, Dehydroepiandrosterone
Type of Study: Original |
Subject:
Behavioral Neuroscience
Received: 2020/10/22 | Accepted: 2020/12/26 | Published: 2022/09/11
Received: 2020/10/22 | Accepted: 2020/12/26 | Published: 2022/09/11
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