Volume 13, Issue 2 (March & April 2022)
BCN 2022, 13(2): 207-214 |
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Bagheri Z, Shamsi F, Zeraatpisheh Z, Salmannejad M, Soltani A, Aligholi H. The Effect of Different Concentrations of Methylprednisolone on Survival, Proliferation, and Migration of Neural Stem/Progenitor Cells. BCN 2022; 13 (2) :207-214
URL: http://bcn.iums.ac.ir/article-1-1687-en.html
URL: http://bcn.iums.ac.ir/article-1-1687-en.html
Zohreh Bagheri1 
, Fatemeh Shamsi2 , Zahra Zeraatpisheh2 , Mahin Salmannejad3 , Ahmad Soltani4 , Hadi Aligholi * 2
, Fatemeh Shamsi2 , Zahra Zeraatpisheh2 , Mahin Salmannejad3 , Ahmad Soltani4 , Hadi Aligholi * 2
1- Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Neurosciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Neurosurgery, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Neurosciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Neurosurgery, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract:
Introduction: The present study addressed whether methylprednisolone (MP) as an anti-inflammatory drug used in neurodegenerative diseases and neural stem/progenitor cells (NS/PCs) is safe.
Methods: First, embryonic rat NS/PCs were exposed to different concentrations of MP, and then we evaluated their survival by MTT assay, proliferation by analyzing the number and diameter of neurospheres, and the migration of the cells by neurosphere assay.
Results: The viability of NS/PCs was reduced following exposure to 10, 15, and 20 µg/mL of MP. In addition, although the number of neurospheres did not change, exposure to different concentrations of MP resulted in the formation of smaller neurospheres. Despite these undesirable effects, the highest concentration of MP (20 μg/mL) increased the migration capacity of the NS/PCs.
Conclusion: The combination of MP and NS/PCs is not recommended due to the adverse effects of MP on the survival and proliferation of NS/PCs.
Methods: First, embryonic rat NS/PCs were exposed to different concentrations of MP, and then we evaluated their survival by MTT assay, proliferation by analyzing the number and diameter of neurospheres, and the migration of the cells by neurosphere assay.
Results: The viability of NS/PCs was reduced following exposure to 10, 15, and 20 µg/mL of MP. In addition, although the number of neurospheres did not change, exposure to different concentrations of MP resulted in the formation of smaller neurospheres. Despite these undesirable effects, the highest concentration of MP (20 μg/mL) increased the migration capacity of the NS/PCs.
Conclusion: The combination of MP and NS/PCs is not recommended due to the adverse effects of MP on the survival and proliferation of NS/PCs.
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2020/01/7 | Accepted: 2020/07/26 | Published: 2022/03/1
Received: 2020/01/7 | Accepted: 2020/07/26 | Published: 2022/03/1
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