Volume 12, Issue 3 (May & June 2021)
BCN 2021, 12(3): 315-324 |
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Sanooghi D, Amini N, Azedi F, Bagher Z, Parvishan A, Lotfi A, et al . Differentiation of Mesenchymal Stem Cells Derived From Human Adipose Tissue Into Cholinergic-like Cells: An in Vitro Study. BCN 2021; 12 (3) :315-324
URL: http://bcn.iums.ac.ir/article-1-1589-en.html
URL: http://bcn.iums.ac.ir/article-1-1589-en.html
Davood Sanooghi1 
, Naser Amini2 , Fereshteh Azedi2 , Zohreh Bagher3 , Asghar Parvishan4 , Abolfazl Lotfi5 , Nooshin Rashidi2 , Erfan Lotfi6 , Forough Azam Sayahpour7 , Faezeh Faghihi * 2
, Naser Amini2 , Fereshteh Azedi2 , Zohreh Bagher3 , Asghar Parvishan4 , Abolfazl Lotfi5 , Nooshin Rashidi2 , Erfan Lotfi6 , Forough Azam Sayahpour7 , Faezeh Faghihi * 2
1- Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
3- ENT and Head & Neck Research Center, The Five Basic Sensory Institute, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
4- Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
5- Damavand Agricultural College, Technical and Vocational University, Tehran, Iran.
6- School of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
7- Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research, Tehran, Iran.
2- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
3- ENT and Head & Neck Research Center, The Five Basic Sensory Institute, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
4- Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
5- Damavand Agricultural College, Technical and Vocational University, Tehran, Iran.
6- School of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
7- Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research, Tehran, Iran.
Abstract:
Introduction: Cholinergic-associated diseases currently constitute a significant cause of neurological and neurodegenerative disabilities. As the drugs are not efficient in improving the suffered tissues, stem cell treatment is considered an effective strategy for substituting the lost cells.
Methods: In the current study, we set out to investigate the differentiation properties of human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) into cholinergic-like cells by two morphogens of Retinoic Acid (RA) and Sonic Hedgehog (Shh) using a three-step in vitro procedure. The results were evaluated using real-time PCR, flow cytometry, and immunocytochemistry for two weeks.
Results: Our data showed that the cells could express cholinergic specific markers, including Islet-1, Acetylcholinesterase (AChE), SMI-32, and Nestin, at mRNA and protein levels. We could also quantitatively evaluate the expression of Islet-1, AChE, and Nestin at 14 days post-induction using flow cytometry.
Conclusion: Human AD-MSCs are potent cells to differentiate into cholinergic-like cells in the presence of RA and Shh through a three-step protocol. Thus, they could be a suitable cell candidate for the regeneration of cholinergic-associated diseases. However, more functional and electrophysiological analyses are needed in this regard.
Methods: In the current study, we set out to investigate the differentiation properties of human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) into cholinergic-like cells by two morphogens of Retinoic Acid (RA) and Sonic Hedgehog (Shh) using a three-step in vitro procedure. The results were evaluated using real-time PCR, flow cytometry, and immunocytochemistry for two weeks.
Results: Our data showed that the cells could express cholinergic specific markers, including Islet-1, Acetylcholinesterase (AChE), SMI-32, and Nestin, at mRNA and protein levels. We could also quantitatively evaluate the expression of Islet-1, AChE, and Nestin at 14 days post-induction using flow cytometry.
Conclusion: Human AD-MSCs are potent cells to differentiate into cholinergic-like cells in the presence of RA and Shh through a three-step protocol. Thus, they could be a suitable cell candidate for the regeneration of cholinergic-associated diseases. However, more functional and electrophysiological analyses are needed in this regard.
Keywords: Cholinergic differentiation, Adipose-derived mesenchymal stem cells, Retinoic acid, Sonic hedgehog
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2019/08/23 | Accepted: 2020/02/15 | Published: 2021/05/1
Received: 2019/08/23 | Accepted: 2020/02/15 | Published: 2021/05/1
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