Volume 12, Issue 6 (November & December - in Press 2021)                   BCN 2021, 12(6): 0-0 | Back to browse issues page

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haghani S, Jamali-Raeufy N, Baluchnejadmojarad T, Roghani M, Aryan L, Fahanik-Babaei J. Hepatocyte Growth Factor Attenuates the Severity of Status Epilepticus in Kainic Acid-Induced Model of Temporal Lobe Epilepsy by Targeting Apoptosis and Astrogliosis. BCN. 2021; 12 (6)
URL: http://bcn.iums.ac.ir/article-1-1562-en.html
Objectives: Although drug therapy is the most common treatment for epilepsy, proper seizure control is not achieved with current medications. This study evaluated the protective effects of the Hepatocyte Growth Factor (HGF) in a rat model of Temporal Lobe Epilepsy (TLE) and explored possible molecular mechanisms.
Methods: A TLE rat model was determined using an intra-hippocampal kainic acid injection (4 μg). Intra-cerebrovascular injection of HGF (6 μg) was performed 30 min before injection of kainic acid.  Learning and memory impairment were investigated by behavioral tests. ELISA assay was used to determine astrogliosis and DNA fragmentation. Changes in neuronal density and mossy fiber sprouting were evaluated by Nissl and Timm staining, respectively.
Results: Behavioral assessments indicated that kainate-treated rats showed spontaneous seizure, and their alternation percentage scores in the Y-Maze test were lower (P<0.001). Likewise, the passive avoidance test confirmed learning disability in Kainate-treated rats (P <0.001). HGF administration reduced the number of spontaneous seizures, alternation percentage score (P<0.001), and cognitive disturbances (P<0.001). The histopathological results also showed that a protected HGF administration contributed to the reduction of neuronal loss in the CA3 subregion of the hippocampus and inhibited the formation of aberrant mossy fiber sprouting (MFS) (P<0.01).  Also, the ELISA assay showed a significant decrease in GFAP (P<0.01) and DNA fragmentation (P<0.05) following HGF administration.
Conclusion: Our findings demonstrate the validity of HGF in protection against progression of the kainate-induced TLE in rats by improving learning, cognitive disturbances, and inhibiting apoptosis and astrogliosis.
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2019/07/21 | Accepted: 2020/08/25 | Published: 2021/09/19

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