1. Introduction
One of the issues facing human societies, whether industrial or nonindustrial, is the irreparable damage to the peripheral nervous system (PNS). Studies have reported that, in Mumbai, damages to the PNS have been reported in 2.4% of people and 8% of individuals over 55 (Azhary, Farooq, Bhanushali, Majid, & Kassab, 2010; Hughes, 2002; Martyn & Hughes, 1997).
However, different therapeutic methods have been adopted for peripheral nerve repair; still, 50% of these damages become permanent and cause disability. However, unlike the Central Nervous System (CNS), peripheral nerve fibers can regenerate and innervate distal targets, a process that starts almost immediately after the damage. Knowing this, it can be hypothesized that understanding the basic regulatory mechanisms for axon regeneration intended to regulate neuronal growth may help introduce new methods to accelerate and enhance nerve regeneration (Samuels, Ropper, & Klein, 2014). 
Dates have played a major role in human life since 7000 years ago, especially for the Arabs. They grow in arid and semi-arid regions (Kanarek, Mandillo, & Wiatr, 2001). According to the Food and Agriculture Organization of the United Nations (FAO), the production, use, and industrial application of dates are rising (Tandon, Srivastava, Nagpal, Khosla, & Singh, 2000). Dates are mostly produced in Egypt, followed by Saudi Arabia, Iran, UAE, and Algeria (Chandrasekaran & Bahkali, 2013). Date palms are cylindrical unbranched trunks, and the entire length of the stem is also free of leaves. They only let out large leaves and pinnate leaflets on the crown at the top. Date palms are in the palm family ‘Palmacae,’ native to Iran, and found in relatively tropical regions of Kermanshah, Khuzestan, Fars, Kerman, Hormozgan, and Sistan and Baluchestan. Dates most likely have medicinal properties that are still unknown (Cook & Furr, 1952). Recent reports concern the antioxidative, liver protection, anti-mutagenic, anti-tumoral, anti-inflammatory, anti-bacterial, and, probably, anti-diarrheal properties as well as protection of the digestive system for this plant (Allaith, 2009; Chevallier, 1996; Hammad & Sallal, 2002; Saafi et al., 2011). Date palms are in the palm family ‘Palmacae,’ bearing the scientific name Phoenix dactylifera (Samuels, et al, 2014).
Dates are used in traditional medicine for curing hoarseness, paralysis, backache, and rheumatic pains, among others. Dates are highly important in our nutrition. The importance of dates is derived from their rich composition: carbohydrates (make up 50%-60% of the dried fruits) (Alkaabi et al., 2011), salts and minerals, dietary fiber, vitamins, fatty acids, amino acids, and proteins. Accordingly, from many aspects, dates are considered an ideal meal. Dates also boost various useful properties, including antioxidative, anti-mutagenic, anti-tumoral, anti-bacterial, and digestive system protection effects (Chevallier, 1996; Hammad & Sallal, 2002). They also have protective effects on the neurons, owing to compounds like polyphenol and melatonin (Vayalil, 2002). The anti-hepatotoxic effects of date extract on rat liver have already been studied (Zangiabadi et al., 2011). Studies have addressed and approved the antioxidative and anti-mutagenic effects of different species of dates (Allaith, 2009; Saafi et al., 2011). There have been studies on the positive antioxidative effects of date extract in preventing diabetic neuropathy. In a study date extract treatment shows efficacy for preventing diabetic deterioration and for improving pathological parameters of diabetic neuropathy in rats, as compared with control groups (Mansouri, Embarek, Kokkalou, & Kefalas, 2005; Shabani, Zangiabadi, & Asadi-Shekaari, 2013). A study on the effects of the watery extract on pain in rats has reported increased pain. 

2. Materials and Methods 
Animals and lab protocols
Care of laboratory animals was based on the Guide for the Care and Use of Laboratory Animals at Kerman Neuroscience Research Center of Kerman University of Medical Sciences, approved by the Animal Ethics Committee.
This study used male Wistar rats weighing 250 to 300 g. The rats received standard diets during the study and were kept in the animal lab under special conditions, away from pathogenic agents at a fixed temperature and normal environment (12:12 hours, light: dark cycle). For statistical accuracy, intensive care was applied to minimize pain in animals and reduce the number of animals.
Animals in this study were divided into control and experimental groups. The control group received daily food and water without date extract, and the test group received food and water plus 10% date extract, 4 mL/kg for three weeks in the daily water intake. Both at the beginning and the end of the intervention, the weight of all the animals was measured along with their nerve conduction velocity NCV.

Extracting method
For preparing the watery date extract, after procurement of fresh Mazafati dates from Bam orchards, the pits were removed, then 200 g of the date flesh was soaked in 2000 mL of distilled water for 48 hours, and later on, completely mixed by a mixer. The resulting mix was centrifuged at 4000 rpm for 20 min, at 4°C. Then, the solution in the upper area of the tube was removed from the remaining sediment and kept at -20°C until further use. By conducting electrodiagnostic assessments, data collection on the control and experimental rats was done by a person blind to the experimental conditions.

NCV assessment and recording
Three weeks after commencing the oral intake of date extract, the animals were anesthetized using ketamine/xylazine (50/20 mg/kg) solution. The environmental temperature was set to 25°C±1°C throughout the study. After shaving the legs of the animals using bipolar electrodes of AD-Instruments ML856 Power-Lab, the sciatic nerve was stimulated at the knee and ankle, and immediately after the stimulation, the muscle’s nerve potential was recorded by the unipolar electrodes attached to the sole of the hind paw. The obtained records were the biphasic responses with an initial M wave created by the stimulation of motor fibers.
NCV (Mean±SD) was calculated as the ratio of the distance between the two stimulation locations (mm) to the recording time difference between the two stimulated regions (m/s). Latency (Mean±SD) was calculated as the time stimulation to recording (millisecond). The height of waves (Mean±SD) was calculated to millivolts. The findings analyzed by SPSS software .

3. Results
The experiment was conducted on 24 male Wistar rats. The Mean±SD weights of the rats were 268.3±8.3 gr prior to and 275.8±11.6 g after date extract intake, which is not significant (P=0.075).
The control group, not receiving date extract, had an Mean±SD weight of 266.25±10.2 gr at the beginning and 279.5±11.7 gr at the end of the experiment, yet, although significant, the weight gain difference between the two groups was not significant (P=1) (Table 1).

NCV significantly increased from 47.2±11.5 m/s before date extract intake to 54.1±15.2 m/s afterward (P<0.001). NCV also increased in the control group: 37.2±7.6 m/s at the beginning of the project and 39.6±8.5 m/s at the end, indicating a significant difference (P=0.001). The increased NCV examined in the two groups, i.e., 2.4±2 m/s in the control group and 6.5±4.1 m/s in the test group shows a significant difference (P=0.008) (Figure 1).

The difference in distal motor latency (DML) in knee and ankle of the cases of test and control groups at the beginning and end of the experiment was not significant (P=0.473) (Table 2).

The height of the waves in the ankle of the control group was 2.3±2.2 mV at the beginning and 2.8±2 mV at the end (P=0.214). The height of the waves n the knees of the control group animals was 1.6±1 mV at the beginning and 1.8±1 mV at the end (P=0.267). On the other hand, the height of the waves in the ankle of the test group animals was 2.3±2.1 mV at the beginning and 5.3±2.4 mV at the end (P=0.001). The height of the waves in the knee of test group animals was 2.9±2.6 mV at the beginning and 4±1.2 mV at the end (P=0.181). The difference in the height of waves in the ankles of animals in the control and test group was significant: 0.8 for the control and 3 for the test group (P=0.020). The difference in the height of waves in the knees of animals in the control and test groups was also significant: 0.4 for the control and 1.1 for the test group (P=0.001) (Figure 2).

4. Discussion
According to our investigations, this study is the first to address the effects of date extract on NCV. The results of the present study show for the first time that watery date extract considerably increases NCV. Furthermore, our results suggest that these effects are induced by accelerated myelination in the neurological unit. Conduction velocity in the CNS and PNS is an agent of myelination, as NCV is slower in unmyelinated fibers than in the myelinated ones (Chevallier, 1996). Myelin is a multi-layer protein and lipid composition formed by the glial cell plasma membrane.
Unlike nerve cells, the remyelination speed is fast and a matter of minutes, independent of the growing age, accelerated myelination. Also, increased myelin diameter is effective in a higher NCV (Vayalil, 2002).

In this study, the authors observed an increased wave height in the ankles of subjects in the test group after intake of watery date extract, which can be due to an increased myelin sheath activity, though this increase is mostly owed to the increased axonal activity.
The two latter findings are derived from the enhanced neural activity in transmitting electrical signals from neurons to the muscle. An activity that is an agent of axonal diameter, which may change drastically in three weeks, and is more related to increased myelin thickness or activity.
The effect of myelin in signal transmission is realized through several major channels. The myelin sheath insulates the axon and inhibits current leakage out of the neural route and, thus, keeps signal strength from weakening. Further, there are the nodes of Ranvier in the periodic gaps in the myelin, which act as springboards in transmitting the signals. Date extract compositions may also accelerate the ionic activity (Allaith, 2009).
The other activities of date palm fruit are effect on bile and fatty acids. In two studies establish that an extract made from date palm fruit acts as a co-agonist ligand for Farnesoid X Receptor (FXR), a nuclear receptor critical for maintaining bile acid, cholesterol, and triglyceride homeostasis (Stroeve et al., 2010; Zhang & Edwards, 2008). In other study shows that dates contain bioactive compounds which exert FXR-mediated regulatory effects that may contribute to the underlying molecular mechanism involved in the triglyceride-lowering action of dates. Additionally, this study identifies a new potential intestinally-mediated mechanism by which poorly-bioavailable polyphenols from dates could affect blood lipid levels without being absorbed systemically (Alfaro-Viquez et al., 2018).
Watery date extract can affect myelin repair and growth. Making up almost half of the date sugar, fructose affects the creation of fat storage in prone areas, also, owing to the many fibers of the date, it can dispose of cholesterol (Zangiabadi et al., 2011). Myelin can be one of these appropriate sites. It is a lipid tissue composed of components with rapid regenerative and rotating capabilities, in some cases, within a few minutes. It may also affect the ion transmission of the nodes of Ranvier (Alkaabi et al., 2011).
The authors observed a significant increase in NCV in the control group, far less than in the test group. This increase is due to the growth of the myelin sheath, which is never-ending, though slows down after puberty. Increased NCV in the control group one month later is justifiable, as myelin synthesis is independent of age (Vayalil, 2002).
In mild lesions of the PNS, initial unpleasant pains are often observed spontaneously or in response to non-painful sensory stimulation (Alkaabi et al., 2011). We believe the impaired transmission of neural signals induces this unpleasant feeling. This condition can be treated using proper medications, which help improve the activity of the PNS, both in the myelin sheath and the axon, and probably prevent the progress of such lesions (Shekaari et al., 2008).

5. Conclusion
The compositions of date extract accelerate electrical signal transmission. 

Ethical Considerations
Compliance with ethical guidelines
The current project was approved by the Neurology Research Center, Kerman University of Medical Sciences. 

Funding
This research did not receive any grant from funding agencies in the public, commercial, or non-profit sectors. 

Authors' contributions
All authors equally contributed to preparing this article.

Conflict of interest
The authors declared no conflict of interest.

Acknowledgments
I would like to extend my appreciation to those who assisted us in all the stages of the project, Mohammad Sheybani, Sheikhshoaei, Jam, and all the kind staff of Kerman Neuroscience Research Center. 


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