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Mahsa Golmohammadi1 
, Atarodalsadat Mostafavinia1 , Mohammad Mahdi Nazarnejad1 , Zahra Nadia Sharifi *1
, Atarodalsadat Mostafavinia1 , Mohammad Mahdi Nazarnejad1 , Zahra Nadia Sharifi *1
1- Department of Anatomical Sciences & Cognitive Neuroscience, Faculty of Medicine, Tehran Medical sciences, Islamic Azad university, Tehran, Iran.
Abstract:
Background: Cerebral ischemia is one of the leading causes of global mortality and the prominent cause of disability in many countries. Damage caused by reperfusion is due to the inflammatory function of the damaged tissue. Ischemia-reperfusion causes the formation of oxygen free radicals and other oxidants. The CA1 region of the hippocampus is one of highly sensitive parts of the brain against ischemia and hypoxia. The Nigella Sativa plant with its antioxidant properties can remove free radicals and Cell damage compounds in order to prevent damage to viable cells and cell death.
Purpose: To determine the effects of Hydroalcoholic Extract and Nano Hydroalcoholic Extract Containing Nigella Sativa on CA1 Region of Hippocampus in Male Wistar Rat Following Transient Global Ischemia / Reperfusion.
Methods: Four groups of 32 male Wistar rats were randomly formed (control, Ischemia, hydroalcoholic extract of black recipient seed and nano-extract of hydroalcoholic black seed recipient groups). Ligation of bilateral common carotid arteries induced Ischemia model. After behavioral test, brains removal was completed and prepared for Nissl staining and soteriological evaluations along with expression level of Bax and Bcl2, by real time PCR technique.
Results: considerable raise in the number of viable pyramidal cells was observed in Hydroalcoholic Extract and Nano Hydroalcoholic Extract groups compared to the ischemia group. Bax expression was elevated and the expression of Bcl-2 was declined subsequent to transient global I/R in the CA1 region. The effects of transient global I/R on genes expression was reversed by the injection of hydroalcoholic and nano-hydroalcoholic extract containing Nigella Sativa.
Conclusion: Transient global I/R remarkably promotes cell death and morphological changes.it seems that Hydroalcoholic Extract of Nigella sativa especially Nano Hydroalcoholic extract of Nigella sativa at a dose of 400 mg / kg, may be a suitable solution for the treatment of ischemia-induced brain damage.
Purpose: To determine the effects of Hydroalcoholic Extract and Nano Hydroalcoholic Extract Containing Nigella Sativa on CA1 Region of Hippocampus in Male Wistar Rat Following Transient Global Ischemia / Reperfusion.
Methods: Four groups of 32 male Wistar rats were randomly formed (control, Ischemia, hydroalcoholic extract of black recipient seed and nano-extract of hydroalcoholic black seed recipient groups). Ligation of bilateral common carotid arteries induced Ischemia model. After behavioral test, brains removal was completed and prepared for Nissl staining and soteriological evaluations along with expression level of Bax and Bcl2, by real time PCR technique.
Results: considerable raise in the number of viable pyramidal cells was observed in Hydroalcoholic Extract and Nano Hydroalcoholic Extract groups compared to the ischemia group. Bax expression was elevated and the expression of Bcl-2 was declined subsequent to transient global I/R in the CA1 region. The effects of transient global I/R on genes expression was reversed by the injection of hydroalcoholic and nano-hydroalcoholic extract containing Nigella Sativa.
Conclusion: Transient global I/R remarkably promotes cell death and morphological changes.it seems that Hydroalcoholic Extract of Nigella sativa especially Nano Hydroalcoholic extract of Nigella sativa at a dose of 400 mg / kg, may be a suitable solution for the treatment of ischemia-induced brain damage.
Keywords: Hydroalcoholic extract, Hippocampus, Ischemia-reperfusion, Nigella Sativa, Nano-hydroalcoholic extract
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2025/03/6 | Accepted: 2025/06/10
Received: 2025/03/6 | Accepted: 2025/06/10
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