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1- Department of physiology, Faculty of Veterinary Medicine, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2- CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
3- Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:  
Orexin-containing neurons originate from the lateral hypothalamic, sending their projections toward mesolimbic regions such as the ventral tegmental area (VTA). In the current study, the reinstatement model has been used to examine the effects of intra- VTA administration of SB334867 as an orexin-1 receptor (OX1R) antagonist on drug priming- and forced swim stress (FSS)-induced reinstatement of morphine. Eighty-eight adult male albino Wistar rats weighing 200 g–280 g were bilaterally implanted by cannulas into the VTA. For induction of conditioned place preference (CPP), subcutaneous (sc) injection of morphine (5 mg/kg) was used daily during a 3-day conditioning phase. Then,  the CCP score  was calculated. After a 24 hr “off” period following achievement of extinction criterion, rats were tested for drug priming-induced reinstatement by priming dose of morphine (1 mg/kg, sc) and for FSS-induced reinstatement 10 min after FSS. In the next experiments, animals received different doses of intra-VTA administration of SB334867 (0.3, 3 and 1 µg/0.3 µl 12% DMSO per side) bilaterally and were subsequently tested for FSS- and morphine priming- induced reinstatement. Our findings indicated that the FSS could induce the reinstatement of seeking behaviors. Furthermore, intra-VTA administration of OX1R antagonists suppressed FSS- and drug priming-induced reinstatement dose-dependently. It is concluded that FSS and drug priming-induced reinstatement may be mediated, at least in part, by stimulation of orexin receptors in the VTA.
Type of Study: Original | Subject: Behavioral Neuroscience
Received: 2018/01/8 | Accepted: 2018/02/24

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