دوره 13، شماره 5 - ( 6-1401 )
جلد 13 شماره 5 صفحات 636-625 |
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Moayeri A, Alizadeh R, Ghasemi Hamidabadi H, Nazm Bojnordi M, Niapour A, Hedayatpour A, et al . Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture. BCN 2022; 13 (5) :625-636
URL: http://bcn.iums.ac.ir/article-1-1873-fa.html
URL: http://bcn.iums.ac.ir/article-1-1873-fa.html
Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture. مجله علوم اعصاب پایه و بالینی. 1401; 13 (5) :625-636
چکیده:
Introduction: The induction of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases, including Parkinson disease. This study aims to differentiate HUC-MSCs into dopaminergic neuron-like cells.
Methods: Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography.
Results: Our results showed that dopaminergic neuronal markers’ transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates.
Conclusion: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.
Methods: Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography.
Results: Our results showed that dopaminergic neuronal markers’ transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates.
Conclusion: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.
نوع مطالعه: Original |
موضوع مقاله:
Cellular and molecular Neuroscience
دریافت: 1399/5/6 | پذیرش: 1399/12/17 | انتشار: 1401/6/20
دریافت: 1399/5/6 | پذیرش: 1399/12/17 | انتشار: 1401/6/20
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