Introduction: The administration of 3,4-methylenedioxymethamphetamine (MDMA) or ecstasy causes memory impairment, whereas neurogenesis improves memory and learning. Hence, this study evaluated the effects of MDMA on neurogenesis in the hippocampus of male rats.
Methods: Adult male Wistar rats received Intraperitoneal (IP) injections of MDMA (10 mg/kg). We assessed nestin, sex-determining region Y-box 2 (Sox2), and NeuroD expressions according to the immunohistochemistry analyses. 
Results: MDMA reduced the expressions of nestin, Sox2, and NeuroD compared with the control groups. The reduction in NeuroD expression was age-related. 
Conclusion: MDMA possibly has negative effects on neurogenesis, which specifically results from impaired survival of newborn cells.