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چکیده:  
Introduction: Application of deep brain stimulation (DBS) has been considered as a new therapeutic manner for neurological disorders, including epilepsy, treatment. However, the precise procedures underlying the anticonvulsant action of high-frequency stimulation (HFS) remain unclear. In this study, we explored the impact of applying high-frequency stimulation in the olfactory bulb on alterations observed in spontaneous excitatory postsynaptic currents (sEPSCs) in kindled animals.
Methods: Male rats underwent a kindling procedure involving semi-rapid electrical stimulation (six stimulations/day) of the dorsal CA1 area. HFS (130 Hz) was administered in full kindled rats at four times (i.e., 5 min, 6 h, 24 h, and 30 h) after the 6th (i.e., the last) kindling stimulation (kindled+HFS group). Subsequently, the impact of HFS on EPSCs was evaluated in pyramidal cells in CA1 area of the hippocampal slices from kindled subjects using whole cell patch clamp technique.
Results: In kindled animals sEPSC amplitude increased (p<0.001), while the sEPSC inter-event interval decreased compared to the control group (p<0.05). Application of HFS in the olfactory bulb of fully kindled rats resulted in a reduction in sEPSCs amplitude and an increase in the sEPSCs’ inter-event interval. There was not any significant difference in membrane potential and input resistance in kindled animals compared to control group. Notably, application of HFS in kindled animals restored the observed changes, so that no significant change was observed in mentioned parameters when kindled+HFS compared with the control group.
Conclusion: These findings suggested that the olfactory bulb may be considered a viable DBS target in epilepsy treatment, and HFS application may mitigate the increase in sEPSCs occurrence following seizure in kindled animals.

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نوع مطالعه: Original | موضوع مقاله: Cellular and molecular Neuroscience
دریافت: 1403/2/18 | پذیرش: 1403/5/7

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