چکیده:
Purpose: In the present study, the culture of embryonic spinal motor neurons (SMNs) was used to assess the impacts of adrenomedullin (AM) on neurotoxic effects of doxorubicin (DOX).
Materials and methods: To prepare the culture of rat embryonic SMNs, spinal cords were isolated from the rat embryos, digested enzymatically, and triturated to obtain spinal cell suspension. Then, the SMNs were purified from the cell suspension using a single gradient of OptiPrep and were cultured. The SMNs were treated with DOX (0.0-100 µM) and AM (3.125-100 nM) and their viability and apoptosis were evaluated using MTT and annexin V flowcytometric assays. Oxidative stress was assessed through the measurement of cellular reactive oxygen species (ROS), nitric oxide (NO), malondialdehyde (MDA), and 8-iso-prostaglandin F2α (iPF2α) levels. Finally, qPCR was employed to determine the expressions of interleukin1-β (IL-1β), inducible NO synthase (iNOS), tumor necrosis factor-α (TNF-α), SRY-related protein 9 (SOX9), matrix metalloproteinase (MMP)-3 and -13.
Results: The viability of SMNs was decreased following DOX treatment dose-dependently (IC50 = 10.54 µM). DOX increased the cellular ROS, MDA, NO, and iPF2α levels (p<0.001). Additionally, AM reduced DOX-induced cell death dose-dependently (p<0.001). AM (50 nM) pretreatment also reduced the DOX-induced oxidative stress (p<0.01) and -genes expression (p<0.01).
Conclusion: Based on the results, AM might be considered a protective factor against chemotherapy-induced toxicity in SMNs.
Materials and methods: To prepare the culture of rat embryonic SMNs, spinal cords were isolated from the rat embryos, digested enzymatically, and triturated to obtain spinal cell suspension. Then, the SMNs were purified from the cell suspension using a single gradient of OptiPrep and were cultured. The SMNs were treated with DOX (0.0-100 µM) and AM (3.125-100 nM) and their viability and apoptosis were evaluated using MTT and annexin V flowcytometric assays. Oxidative stress was assessed through the measurement of cellular reactive oxygen species (ROS), nitric oxide (NO), malondialdehyde (MDA), and 8-iso-prostaglandin F2α (iPF2α) levels. Finally, qPCR was employed to determine the expressions of interleukin1-β (IL-1β), inducible NO synthase (iNOS), tumor necrosis factor-α (TNF-α), SRY-related protein 9 (SOX9), matrix metalloproteinase (MMP)-3 and -13.
Results: The viability of SMNs was decreased following DOX treatment dose-dependently (IC50 = 10.54 µM). DOX increased the cellular ROS, MDA, NO, and iPF2α levels (p<0.001). Additionally, AM reduced DOX-induced cell death dose-dependently (p<0.001). AM (50 nM) pretreatment also reduced the DOX-induced oxidative stress (p<0.01) and -genes expression (p<0.01).
Conclusion: Based on the results, AM might be considered a protective factor against chemotherapy-induced toxicity in SMNs.
نوع مطالعه: Original |
موضوع مقاله:
Cellular and molecular Neuroscience
دریافت: 1400/5/27 | پذیرش: 1401/1/23
دریافت: 1400/5/27 | پذیرش: 1401/1/23
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
