دوره 12، شماره 6 - ( 8-1400 )                   جلد 12 شماره 6 صفحات 744-737 | برگشت به فهرست نسخه ها


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Saadat F, Zareighane Z, Safavifar F, Jalali S Z, Berahmeh A, Khorramizadeh M R. The Repression of Matrix Metalloproteinases and Cytokine Secretion in Glioblastoma by Targeting K+ Channel. BCN 2021; 12 (6) :737-744
URL: http://bcn.iums.ac.ir/article-1-1419-fa.html
The Repression of Matrix Metalloproteinases and Cytokine Secretion in Glioblastoma by Targeting K+ Channel. مجله علوم اعصاب پایه و بالینی. 1400; 12 (6) :737-744

URL: http://bcn.iums.ac.ir/article-1-1419-fa.html


چکیده:  
Introduction: Glioblastoma is an aggressive human brain malignancy with poorly understood pathogenesis. Voltage-gated potassium (Kv) channels and Matrix Metalloproteinases (MMPs) are highly expressed in malignant tumors and involved in the progression and metastasis of glioblastoma. This study aimed to determine whether a voltage-dependent potassium channel blocker could modulate astrocytes as a cell involved in the immunopathogenesis of glioblastoma. 
Methods: The cytotoxic effect of 4-Aminopyridine (4-AP) at different doses in the cell model of glioblastoma was measured by MTT assay. The ELISA technique and gelatin zymography were used to assess cytokine levels and MMP-9 after 4-AP treatment. 
Results: Cytotoxicity analysis data indicated that cell viability reduced by increasing 4-AP level and cell growth decreased gradually by removing 4-AP from the cell medium. 4-AP inhibits the secretion of IL-6 and IL-1 (P<0.05). MMP9 activity significantly inhibits with increased 4-AP dose, compared to non-treated cells.
Conclusion: The reduction of cell viability, IL-6 secretion, and MMP-9 activity in an in vitro model of glioblastoma might be assumed 4-AP as an agent for chemoprevention of cancer.
نوع مطالعه: Original | موضوع مقاله: Cellular and molecular Neuroscience
دریافت: 1397/11/11 | پذیرش: 1399/3/26 | انتشار: 1400/8/10

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