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1- Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2- Neurophysiology Research Center, Shahed University, Tehran, Iran.
Abstract:  

Kainate (KA) is an agonist for a subtype of ionotropic glutamate receptor. KA can induce neuronal overactivity and excitotoxicity. Rosmarinic acid (RA) is a natural polyphenolic compound with antioxidant, anti-apoptotic, anti-neurodegenerative, and anti-inflammatory properties. We designed this study to assess the effect of RA on apoptosis, nNOS-positive neurons number, and COX-2  and MAPK immunoreactivity, following intrahippocampal kainate in the rat. Rats were randomly assigned to three groups Sham, Kainate (KA was injected into the right side of the hippocampus) and Kainate+ RA (dose of 10 mg/kg/day through a gavage needle for 1 week before injected of KA). Then, histopathological changes included apoptosis (TUNEL assay), nNOS-positive neurons number, MAPK and COX-2 immunoreactivity were evaluated in the hippocampus. In the RA pretreated group, nNOS-positive neurons and TUNEL- positive cells were significantly reduced compared to Kainate group (p<0.05). MAPK and COX-2 immunoreactivity showed no significant changes as compared to Kainate group and a significant higher reactivity for COX-2 (p<0.01) and MAPK (p<0.005) versus sham. In Conclusion RA has neuroprotective effect against KA through reduced apoptosis and nNOS-positive neurons but not MAPK and COX-2.

Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2016/04/12 | Accepted: 2018/10/27

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