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1- Ms.c Student
2- B.Sc. of Biology
3- B.Sc. of Laboratory Sciences
4- Professor of Neurology
5- M.Sc. of Immunology
6- Ph.D student
7- Professor of Immunology
Abstract:  

Introduction: Multiple sclerosis (MS) is an inflammatory disorder caused by self-reactive Th1 lymphocytes, while Th2 cells may confer protection. The Th1- and Th2 cell differentiation is regulated by specific transcription factors especially T-bet and GATA-3, respectively. This investigation aimed to measure the T-bet and GATA-3 expression by peripheral blood mononuclear cells (PBMCs) obtained from MS patients after specific and non-specific in vitro stimulation.
Material and Methods: The PBMCs were separated from 22 patients with MS and 20 healthy individuals, and cultured at 37 °C for 24 h in the absence of a stimulator or in the presence of MOG or PHA at a concentration of 10 μg/ml. Then the T-bet and GATA-3 expression was measured by real time-PCR.
Results: The T-bet expression was enhanced, while the GATA-3 expression was diminished, and therefore the expression of T-bet/GATA-3 ratio was diminished in not-stimulated-, MOG-stimulated and PHA-induced PBMCs from MS patients compared with equal cultures from the healthy individuals (P<0.01, P<0.01 and P<0.01, for T-bet; P<0.03, P<0.01 and P<0.02, for GATA-3 ; P<0.01, P<0.001 and P<0.01 for T-bet/GATA-3 ratio, respectively). The not-stimulated-, MOG-stimulated and PHA-stimulated PBMCs from men with MS expressed higher amounts of GATA-3 than equal cells from MS women (P<0.05, P<0.05 and P<0.01, respectively).
Conclusion: These results probably indicate an imbalance in Th1/Th2 cells in level of transcription factors with a tendency toward Th1 cells in MS. The clinical utilization of the transcription factors as novel biomarkers of MS should be evaluated in further studies.
 

Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2016/03/10 | Accepted: 2018/07/12

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