Volume 9, Issue 6 (November & December 2018)                   BCN 2018, 9(6): 458-469 | Back to browse issues page


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Etesam Z, Nemati M, Ebrahimizadeh M, Ebrahimi H, Hajghani H, Khalili T et al . Different Expressions of Specific Transcription Factors of Th1 (T-bet) and Th2 cells (GATA-3) by Peripheral Blood Mononuclear Cells From Patients With Multiple Sclerosis. BCN. 2018; 9 (6) :458-469
URL: http://bcn.iums.ac.ir/article-1-744-en.html
1- Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
2- Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
3- Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
4- Department of Immunology, School of Medical, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Abstract:  

Introduction: Multiple Sclerosis (MS) is an inflammatory disorder caused by self-reactive Th1 lymphocytes, while Th2 cells may confer protection. The Th1 and Th2 cell differentiation are regulated by specific transcription factors, especially T-bet and GATA-3, respectively. This investigation aimed to measure the T-bet and GATA-3 expression by Peripheral Blood Mononuclear Cells (PBMCs) obtained from MS patients after specific and non-specific in vitro stimulation.
Methods: The PBMCs were separated from 22 patients with MS and 20 healthy individuals. They were cultured at 37°C for 24 h in the absence of a stimulator or in the presence of Myelin oligodendrocyte Glycoprotein (MOG) or Phytohemagglutinin (PHA) at a concentration of 10 μg/mL. Then the T-bet and GATA-3 expression was measured by real time-PCR.
Results: The T-bet expression was enhanced, while the GATA-3 expression diminished. Therefore the expression of T-bet/GATA-3 ratio diminished in not-stimulated, MOG-stimulated and PHA-stimulated PBMCs from MS patients compared with equal cultures from the healthy individuals (P<0.01, P<0.01 and P<0.01, for T-bet; P<0.03, P<0.01 and P<0.02, for GATA-3; P<0.01, P<0.001 and P<0.01 for T-bet/GATA-3 ratio, respectively). The not-stimulated, MOG-stimulated, and PHA-stimulated PBMCs from men with MS expressed higher amounts of GATA-3 than equal cells from MS women (P<0.05, P<0.05 and P<0.01, respectively).
Conclusion: These results probably indicate an imbalance in Th1/Th2 cells in the level of transcription factors with a tendency toward Th1 cells in MS. The clinical utilization of the transcription factors as novel biomarkers of MS should be evaluated in further studies.

Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2016/03/10 | Accepted: 2018/04/20 | Published: 2018/11/1

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