Volume 8, Issue 3 (May & June 2017 -- 2017)                   BCN 2017, 8(3): 183-192 | Back to browse issues page

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Piri H, Haghdoost-Yazdi H, Fraidouni N, Dargahi T, Yaghoubidoust M, Azadmehr A. Research Paper: The Anti-Parkinsonism Effects of KATP Channel Blockade in the 6-Hydroxydopamine-Induced Animal Model: The Role of Oxidative Stress . BCN. 2017; 8 (3) :183-192
URL: http://bcn.iums.ac.ir/article-1-652-en.html
1- Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
2- Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
3- Department of Immunology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

Introduction: Studies suggest that ATP-sensitive potassium (KATP) channels are a potential pharmacotherapeutic target for neuroprotection in neurodegenerative diseases. The current study aimed at evaluating the effect of pretreatment with glibenclamide (Glib) and B vitamins supplement on the severity of behavioral symptoms in 6-hydroxydopamine (OHDA)-induced Parkinsonism. Also malondialdehyde (MDA) concentration was measured in the blood and brain suspensions to find probable neuroprotective mechanism of Glib.
Methods: The 6-OHDA was injected into striatum of rats by stereotaxic surgery. Treatment with Glib and B vitamins was started before the surgery and continued up to 3 weeks after that. Development and severity of Parkinsonism were evaluated by conventional behavioral tests. MDA values were measured spectrophotometrically using thiobarbituric acid and MDA standard curve.
Results: Pretreatments with Glib, at both doses of 1 and 5 mg/kg or B vitamins significantly ameliorated severity of the behavioral symptoms. Pretreatment with a combination of Glib and B vitamins was more effective than pretreatment with Glib or B vitamins alone. Also, pretreatment with B vitamins, Glib, or a combination of them reduced MDA concentration in the brain suspensions. Decrease in MDA concentration in the group of rats that received a combination of B vitamins and Glib was more prominent than that of the Glib groups. 
Conclusion: As severity of the behavioral symptoms in the 6-OHDA-induced Parkinsonism reflects the degree of the lesion in Substantia Nigra (SN) dopaminergic neurons, it is suggested that Glib pretreatment has neuroprotective effect against 6-OHDA-induced neurotoxicity. The current study data also showed that this effect may be mediated by antioxidant effect of Glib. 

Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2016/09/6 | Accepted: 2016/12/20 | Published: 2017/05/1

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