Volume 5, Number 1 (Winter 2014 -- 2014)                   BCN 2014, 5(1): 18-21 | Back to browse issues page


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Dinarvand A, Goodarzi A, Vousooghi N, Hashemi M, Dinarvand R, Ostadzadeh F, et al . Mu Opioid Receptor Gene: New Point Mutations in Opioid Addicts. BCN. 2014; 5 (1) :18-21
URL: http://bcn.iums.ac.ir/article-1-463-en.html

1- Science and Research Branch of Islamic Azad University, Tehran, Iran.
2- Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran.
3- Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
4- Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran
5- Department of Pharmacoeconomics and Pharmaceutical Administration, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
6- Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Abstract:  

Introduction: Association between single-nucleotide polymorphisms (SNPs) in mu opioid receptor gene and drug addiction has been shown in various studies. Here, we have evaluated the existence of polymorphisms in exon 3 of this gene in Iranian population and investigated the possible association between these mutations and opioid addiction. 

Methods: 79 opioid-dependent subjects (55 males, 24 females) and 134 non-addict or control individuals (74 males, 60 females) participated in the study. Genomic DNA was extracted from volunteers’ peripheral blood and exon 3 of the mu opioid receptor gene was amplified by polymerase chain reaction (PCR) whose products were then sequenced. 
Results: Three different heterozygote polymorphisms were observed in 3 male individuals: 759T>C and 877G>A mutations were found in 2 control volunteers and 1043G>C substitution was observed in an opioid-addicted subject. Association between genotype and opioid addiction for each mutation was not statistically significant. 
Discussion: It seems that the sample size used in our study is not enough to confirm or reject any association between 759T>C, 877G>A and 1043G>C substitutions in exon 3 of the mu opioid receptor gene and opioid addiction susceptibility in Iranian population.
Type of Study: Original | Subject: Behavioral Neuroscience
Received: 2012/11/27 | Accepted: 2013/08/13 | Published: 2014/01/1

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