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Sareh Kazmi1 
, Neda Yazdanfar2 , Fatemehsadat Seyedaghamiri2 , Alireza Pishgahi3 , Mehdi Yousefi4 , Tahereh Ghadiri2 , Saeed Sadigh-Eteghad5 , Mehdi Farhoudi *5
, Neda Yazdanfar2 , Fatemehsadat Seyedaghamiri2 , Alireza Pishgahi3 , Mehdi Yousefi4 , Tahereh Ghadiri2 , Saeed Sadigh-Eteghad5 , Mehdi Farhoudi *5
1- Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. & Department of Neuroscience, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
2- Department of Neuroscience, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
3- Physical Medicine and Rehabilitation Specialist, Motahari General Hospital, Isfahan, Iran.
4- Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5- Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2- Department of Neuroscience, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
3- Physical Medicine and Rehabilitation Specialist, Motahari General Hospital, Isfahan, Iran.
4- Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5- Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract:
Globally, stroke ranks as the second most prevalent cause of death, contributing significantly to worldwide mortality burdens, imposing a significant economic and emotional challenge on societies. This study designed to investigate the effect of autologous conditioned serum (ACS) on memory and associated molecular factors in a mouse model of photothrombotic ischemic stroke.
The photothrombotic model was used to induce medial prefrontal cortex (mPFC) ischemia. ACS were prepared by intracardiac puncture of C57BL/6 mice using special ACS syringes. After blood incubation, the sample was centrifuged, and the serum was analyzed with ELISA kits to quantified the levels of IL-1RA and IGF-I. The ischemic animals received 48 µl intranasal ACS in the periods of two times a day, once a day, or once every other day for one week. Behavioral tests, including the Lashley-III maze and social interaction test, were conducted following treatment administration. Additionally, the IGF-1, IL-1β, IL-1RA, levels, and phospho-tau/total-tau ratio were measured in the mPFC area by western blot. Histological analysis was performed to assess ischemic volume.
The results indicated that once-daily ACS administration significantly improved spatial memory in the Lashley-III maze and showed a notable enhancement in social memory as measured by the social interaction test. In terms of molecular analysis, ACS increased the levels of IGF-1 and IL-1RA, whilst decreasing the levels of IL-1β and p-tau/total-tau ratio.
In conclusion, post-stroke intranasal ACS administration enhances memory possibly by increasing the level of IGF-1 and attenuating inflammation through the inhibition of IL-1β signal by IL-1RA, and regulation of tau levels.
The photothrombotic model was used to induce medial prefrontal cortex (mPFC) ischemia. ACS were prepared by intracardiac puncture of C57BL/6 mice using special ACS syringes. After blood incubation, the sample was centrifuged, and the serum was analyzed with ELISA kits to quantified the levels of IL-1RA and IGF-I. The ischemic animals received 48 µl intranasal ACS in the periods of two times a day, once a day, or once every other day for one week. Behavioral tests, including the Lashley-III maze and social interaction test, were conducted following treatment administration. Additionally, the IGF-1, IL-1β, IL-1RA, levels, and phospho-tau/total-tau ratio were measured in the mPFC area by western blot. Histological analysis was performed to assess ischemic volume.
The results indicated that once-daily ACS administration significantly improved spatial memory in the Lashley-III maze and showed a notable enhancement in social memory as measured by the social interaction test. In terms of molecular analysis, ACS increased the levels of IGF-1 and IL-1RA, whilst decreasing the levels of IL-1β and p-tau/total-tau ratio.
In conclusion, post-stroke intranasal ACS administration enhances memory possibly by increasing the level of IGF-1 and attenuating inflammation through the inhibition of IL-1β signal by IL-1RA, and regulation of tau levels.
Keywords: Ischemic Stroke, Autologous conditioned serum, phosphorylated tau, interleukin-1 receptor antagonist, insulin like growth factor-1
Type of Study: Original |
Subject:
Cellular and molecular Neuroscience
Received: 2025/03/3 | Accepted: 2025/05/12
Received: 2025/03/3 | Accepted: 2025/05/12
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