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1- Immunology Department, School of medicine, Isfahan University of medical sciences, Isfahan, Iran.
2- Immunology Department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Neurology Department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: Multiple sclerosis has been considered as chronic inflammation of the central nervous system (CNS) and autoimmune disease .MS is most widely considered to be mediated by activation of myelin-specific T CD4+ cells as well as TH1 and TH17 cells. TH17 cell has been involved in the pathogenesis of MS in various ways. HIF-1α and RORC are required for natural differentiation of TH17 and are essential transcription factors for the evolution of TH17 cells. Numerous studies indicate that epigallocatechin gallate (EGCG) has immunomodulatory and anti-inflammatory effects.
Aims: This study investigated the effect of EGCG on normoxic HIF-1α and RORC2 expression in PBMCs of MS patients.
Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of new cases MS patients. The cells cultured in the presence of a different concentration of EGCG
 (25, 50,100μM) for 18 and 48 hours. Afterward, HIF-1α and RORC2 level expressions were measured by enzyme-linked immunosorbent assay (ELISA) and Real-Time PCR, respectively.
Result: The results showed that EGCG significantly decrease RORC2 gene expression. However, EGCG did not influence the level of HIF-1α. Our present data has led us to conclude that EGCG could be considered as an anti-inflammatory agent may serving as an achievable therapeutic agent for MS.
Subject: Cellular and molecular Neuroscience
Received: 2019/12/5 | Accepted: 2020/04/6 | Published: 2018/03/15

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