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1- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Liver and Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
4- Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
5- Department of Pathology and Clinical Laboratory Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
6- Department of Epidemiology and Biostatistics, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
7- Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
Abstract:  
Multiple sclerosis (MS) is a myelin-degenerating autoimmune disease in the central nervous system (CNS). Experimental autoimmune encephalomyelitis (EAE), due to its clinical and pathologic features similar to multiple sclerosis, is widely used in many studies of this disease as an effective and similar model. Probiotics, comprising lactobacilli, are known to induce immunomodulatory activity with hopeful effects in inflammatory diseases. In this study, the potential of Enterococcus durans and three various strains of lactobacilli (lacto-mix), Including L.rhamnosus, L.casei, and L.plantarum was tested for prevention of EAE features. C57BL/6 female mice were inoculated with (MOG35-55) / (CFA) to induce EAE. Different groups (five groups: n = 6 in each group) of animals received saline or probiotics. Histopathological studies showed an increase in infiltration of inflammatory cells and destruction of the myelin membrane in the EAE group but reduced in the probiotic-treated animals. The concentration of Pro-inflammatory cytokines (IL-17 and IFN-ɣ) in the supernatant of the brain and spinal cord homogenized tissues was significantly increased in EAE compared with the normal saline group. While in the spinal cord, the levels of IL-17 decreased in animals treated with Lacto-mix and Edu + Lacto- mix and a level of IFN-ɣ in animals receiving Edu decreased significantly. In addition, Western blot analysis of MMP-9 and MBP proteins decreased and increased in treatment and EAE groups compared to the normal control group respectively. Collectively, our data indicate that probiotic Enterococcus durans and lacto-mix have a preventive effect on EAE, but more studies are needed to diagnose precise mechanisms and their application in preclinical and clinical trials.


 

Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2019/07/30 | Accepted: 2020/04/6

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