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1- Pasteur Institute of Iran
2- University of Tehran
Alzheimer’s disease (AD) considers as a neurodegenerative disorder containing progressive decrease of memory and other cognitive dysfunctions. The amyloid βeta (Aβ) production and synaptic loss is suppressed by PKCε. In this study, spatial learning and memory for treated rats was evaluated by Morris water maze. The activity (total PKC), mRNA expression, and protein level of PKCe in the platelet and hippocampus tissue were evaluated using immunosorbent assay, Real-time qPCR and western blotting analysis, respectively. Our results showed that traveled distance was significantly prolonged and escape latency significantly decreased in Aβ treated groups. PKC activity assay showed that there was a remarkable difference between Aβ treated and sham operated groups on day 10 and 30 in the hippocampus and also day 30 in platelet after the injection of Aβ. A significant effect in PKC activity between days 0 and 10, days 0 and 30 as well as days 5 and 30 were observed. Amyloid beta significantly down regulated the PKCe mRNA expression in hippocampus of rat on day 30 but no significant difference was observed in platelet. Western blot analysis demonstrated Aβ significantly reduced PKCe protein expression in hippocampus of treated groups on day 30. We concluded; the expression ratio of PKCe was down regulated following the injection of Aβ in the hippocampus, but no significant difference between AD and sham groups were observed in platelet that may be results from the little concentration of PKCe or duration time of Aβ exposed in the rat brain.

Type of Study: Original | Subject: Behavioral Neuroscience
Received: 2018/12/3 | Accepted: 2019/02/7

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