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1- Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
3- Addiction Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
4- Rayan Center for Neuroscience and Behavior, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Abstract:  
Introduction: NUCB2/Nesfatin-1, a newly identified anorexigenic peptide, has antioxidant, anti-inflammatory and anti-apoptotic properties. Brain ischemia/reperfusion induces irreversible damage especially in the hippocampus area. This study was designed to examine the protective effects of NUCB2/nesfatin-1 on expression of apoptosis-related proteins and reactive astrogliosis level in CA1 area of the rat hippocampus after transient global cerebral ischemia.
Methods: The male Wistar rats were randomly allocated into 4 groups (sham, NUCB2/nesfatin-1, ischemia/reperfusion, ischemia/reperfusion+NUCB2/nesfatin-1), (n=7). Cerebral ischemia model prepared by occlusion both common carotid arteries for 20 minutes. Saline as a vehicle and nesfatin-1 at doses 20 µg/kg (intraperitoneally) were injected at the beginning of reperfusion period. Assessment of the proteins expression levels was performed by immunofluorescence and immunohisto chemical staining.
Results: NUCB2/nesfatin-1 significantly reduced the Bax and GFAP proteins level in the CA1 area after ischemia (P<0.05), also NUCB2/nesfatin-1 increased Bcl-2 protein level (P<0.05).
NUCB2/nesfatin-1 exerts protective effects against ischemia injury by inhibition of astrocytes activation as an inflammatory response and decrease neuronal cell apoptosis.
Conclusion: our study for the first time provides the possible clinical view of nesfatin-1in the treatment of cerebral ischemia.
Type of Study: Original | Subject: Behavioral Neuroscience
Received: 2018/05/31 | Accepted: 2018/10/13

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