Volume 11, Issue 3 (May & June 2020)
BCN 2020, 11(3): 261-268 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rezaei F, Saebipour M R, Ghaemi K, Hassanzadeh-Taheri M M, Foadoddini M, Hosseini M. Intra-cerebroventricular Administration of Crocin Attenuates Sleep Deprivation-induced Hyperalgesia in Rats. BCN 2020; 11 (3) :261-268
URL: http://bcn.iums.ac.ir/article-1-1210-en.html
URL: http://bcn.iums.ac.ir/article-1-1210-en.html
Faezeh Rezaei1 
, Mohammad Reza Saebipour * 1, Kazem Ghaemi2 , Mohammad Mehdi Hassanzadeh-Taheri3 , Mohsen Foadoddini4 , Mehran Hosseini3
, Mohammad Reza Saebipour * 1, Kazem Ghaemi2 , Mohammad Mehdi Hassanzadeh-Taheri3 , Mohsen Foadoddini4 , Mehran Hosseini3
1- Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
2- Department of Neurosurgery, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Anatomy, Cellular and Molecular Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
4- Department of Physiology, Cardiovascular Diseases Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
2- Department of Neurosurgery, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Anatomy, Cellular and Molecular Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
4- Department of Physiology, Cardiovascular Diseases Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Abstract:
Introduction: Sleep deprivation can cause hyperalgesia and interfere with analgesic treatments. The aim of the present study was to establish an obligatory sleep-abstinence model and also evaluate the effects of intracerebroventricular (ICV) injection of crocin on pain perception in Wistar rats.
Methods: In this experimental study, 35 adult male Wistar rats were randomly divided into 5 groups (n=7). The intra-ventricular cannulation was done for all rats before sleep deprivation. Sleep deprivation was performed by placing animals on a chamber equipped with an automatic animated conveyor (5 s with an interval of 3 min) for 72 h. Subsequently, the sleep-deprived animals received ICV injection of saline (MOD), Morphine 10 µg (MOR), Crocin 10 ug (Cr10), and Crocin40 µg (Cr40) using a microsyringe. Besides, a non-sleep-deprived group was allocated as a Control Group (NC) and only received an ICV injection of saline. Fifteen minutes after the ICV injections, pain perception was evaluated by the hot plate test (54±0.4◦C).
Results: Compared with the NC group, latency significantly decreased in the MOD group (6.28±0.48 vs. 4.28± 0.48, p<0.0001). In comparison with the MOD group, both morphine (8.42±1.53) and crocin (7.60±1.45 for Cr10 and 8.14±0.89 for Cr40) could significantly increase latency in the sleep-deprived animals (p<0.0001). There was no statistically significant difference between the Cr10 and Cr40 (p=0.42), Cr10, and MOR (p=0.059) and Cr40 with MOR (p=0.86) groups.
Conclusion: Our results indicated that crocin could attenuate hyperalgesia induced by sleep deprivation in rats.
Methods: In this experimental study, 35 adult male Wistar rats were randomly divided into 5 groups (n=7). The intra-ventricular cannulation was done for all rats before sleep deprivation. Sleep deprivation was performed by placing animals on a chamber equipped with an automatic animated conveyor (5 s with an interval of 3 min) for 72 h. Subsequently, the sleep-deprived animals received ICV injection of saline (MOD), Morphine 10 µg (MOR), Crocin 10 ug (Cr10), and Crocin40 µg (Cr40) using a microsyringe. Besides, a non-sleep-deprived group was allocated as a Control Group (NC) and only received an ICV injection of saline. Fifteen minutes after the ICV injections, pain perception was evaluated by the hot plate test (54±0.4◦C).
Results: Compared with the NC group, latency significantly decreased in the MOD group (6.28±0.48 vs. 4.28± 0.48, p<0.0001). In comparison with the MOD group, both morphine (8.42±1.53) and crocin (7.60±1.45 for Cr10 and 8.14±0.89 for Cr40) could significantly increase latency in the sleep-deprived animals (p<0.0001). There was no statistically significant difference between the Cr10 and Cr40 (p=0.42), Cr10, and MOR (p=0.059) and Cr40 with MOR (p=0.86) groups.
Conclusion: Our results indicated that crocin could attenuate hyperalgesia induced by sleep deprivation in rats.
Type of Study: Original |
Subject:
Behavioral Neuroscience
Received: 2018/05/27 | Accepted: 2019/05/6 | Published: 2020/07/14
Received: 2018/05/27 | Accepted: 2019/05/6 | Published: 2020/07/14
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
