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Proteoglycans of extracellular matrix, increases in the glial scar during spinal cord injury and play important role in inhibition of axonal regeneration.
So far, the results of injury therapies have been limited due to lack of identifying the timely therapeutic intervention. The present study aimed to investigate the glial scar chondroitin sulfate (CS) and dermatan sulfate (DS) levels at different times post-injury to determine the appropriate time for therapeutic intervention.
By an experimental study 72 Wistar rats were randomly divided into 12 groups: control, sham, injured animals at 1, 2, 4 and 8 days and 2, 4, 8, 12, 16 and 20 weeks post-injury. Animals in the injured groups were contused in the T10 segment of spinal cord. The motor function of animals was assessed using BBB test and the histological assessment was performed using Luxol Fast Blue and Bielshovisky Staining. CS and DS levels of lesions were measured using ELISA method.
The motor function assessment indicated a relative recovery over time. Histological results confirmed some reregeneration in the injury site at 20 weeks post-injury.  The ELISA results demonstrated much higher level of DS than that of CS in the glial scar.
Considering high levels of DS compared to CS in the glial scar and its reduction from second weeks after SCI onwards, it seems that second week after SCI is the best time for the therapeutic intervention in terms of the scar permeability.
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2018/04/9 | Accepted: 2019/01/14

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