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1- Department of Physiology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2- Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Neurobiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Neurobiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:  
Introduction: Sex differences in outcomes of SCI suggest a sex hormone-mediated effect on post-SCI pathological events including glutamate excitotoxicity. The aim of this study was to  investigate the importance of gonadal hormones on glutamate release subsequent to SCI in rats.
Methods: After laminectomy at T8-T9, an electrolytic lesion was applied on spinothalamic tract in male and female rats. Using spinal microdialysis, we assessed glutamate levels at the site of lesion in both intact and gonadectomized  rats for four hours. Sex differences in the glutamate concentrations was examined. 
Results: The peak retention time of glutamate level was 10.6 minutes and the maximum level of glutamate was determined 40 min post-injury. Gonadectomy of male SCI rats resulted in a significant increase in glutamate concentration (p<0.001) after injury which was a maximum of 40 min after injury as well. But it didn’t cause any significant alterations in gonadectomized female rats.
Conclusion: The significant differences in glutamate levels between both intact and gonadectomised SCI male and female rats, shows the sex hormone-related mechanisms underlying the molecular events in the second phase of SCI. It seems that the role of male's gonadal hormones to prevent the glutamate excitotoxicity is more prominent. The exact mechanisms of these hormones on improvement of functional recovery need to be clarified.
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2018/02/23 | Accepted: 2018/06/26 | Published: 2018/11/28

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