Volume 10, Issue 3 (May & June 2019)                   BCN 2019, 10(3): 225-234 | Back to browse issues page

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Samandari R, Hassanpour-Ezatti M, Fakhri S, Abbaszadeh F, Jorjani M. Sex Differences and Role of Gonadal Hormones on Glutamate Level After Spinal Cord Injury in Rats: A Microdialysis Study. BCN. 2019; 10 (3) :225-234
URL: http://bcn.iums.ac.ir/article-1-1136-en.html
1- Department of Physiology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
2- Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3- Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Introduction: Sex differences in outcomes of Spinal Cord Injury (SCI) suggest a sex-hormone-mediated effect on post-SCI pathological events, including glutamate excitotoxicity. This study aimed to investigate the importance of gonadal hormones on glutamate release subsequent to SCI in rats.
Methods: After laminectomy at T8-T9, an electrolytic lesion was applied to the spinothalamic tracts of male and female rats. Using spinal microdialysis, we assessed glutamate levels at the site of lesion in both intact and gonadectomized rats for 4 hours. In this way, we examined the sex differences in the glutamate concentrations.
Results: The peak retention time of glutamate level was 10.6 min and spinal glutamate concentration reached a maximum level 40 min following SCI. In male SCI rats, gonadectomy caused a significant elevation of glutamate level (P<0.001) following injury which was maximum 40 min post-SCI as well. However, no significant alterations were seen in gonadectomized female rats.
Conclusion: The significant differences in glutamate levels between both intact and gonadectomized SCI male and female rats show the sex-hormone-related mechanisms underlying the molecular events in the second phase of SCI. It seems that the role of male gonadal hormones to prevent glutamate excitotoxicity is more prominent. The exact mechanisms of these hormones on the functional recovery after SCI should be clarified in further studies.
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2018/02/23 | Accepted: 2018/06/26 | Published: 2019/07/21

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