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1- 1. Research Center and Department of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
2- 2. Faculty of nursing, Qom University of Medical Sciences, Qom, Iran.
3- Research Center of Nervous System Stem Cells, Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Abstract:  
Objective: Stem cell therapy has shown ability in recovery of stroke-induced injury in young animal. However, there is not enough data to support efficacy of stem cells in stroke in aged and middle aged rodents. Aim of this study is to assess whether combination bone marrow stromal cells )BMSCs( with thyroid hormone (TH) and mild treadmill exercise can decrease stroke complications in middle-aged mice, as we showed in our previous study in young mice.
Materials and methods: Under Laser Doppler Flowmetry (LDF) monitoring, transient focal cerebral ischemia was prepared by right middle cerebral artery occlusion (MCAO) for 45 min followed by 7days of reperfusion in middle-aged mice. BMSCs (1× 105) were injected into the right cerebral ventricle 24h after MCAO, followed by daily injection of TH (20 µg/100 g/day S.C) and 6 days of running on a treadmill. Infarct size, neurological function, apoptotic cells and expression levels of GFAP (Glial Fibrillary Acidic Protein) were evaluated 1-week post stroke.
Results: Post-ischemic treatment with BMSCs or with TH and/or mild treadmill exercise alone or in combination did not significantly change neurological function, infarct size, and apoptotic cells 7 days after ischemia in middle-aged mice. However the expression of GFAP decreased after treatment with BMSCs and/or TH.
Conclusions: Our findings indicated that BMSCs treatment combined with TH and exercise has no beneficial effects in recovery of stroke injury in middle-aged mice. Comparison between our previous findings in young mice with present findings in middle-aged mice showed that age is not an ignorable variable in studies related to stroke treatment. This study reemphasizes the importance of using aged animals in stroke researches.
 
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2018/01/6 | Accepted: 2018/06/12

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