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1- Department of Physiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
Introduction: Ziziphora clinopodioides has been used widely for various therapeutic purposes in Iranian folk medicine. The aim of the current study was to determine interaction of antinociceptive effect of the essential oil of Ziziphora clinopodioides (EOZC) and opioidergic system in male rats using formalin test.
Methods: Sixty-four male Wistar rats were distributed into eight groups. The groups 1-7 were injected with saline, vehicle (Tween-80, 0.5%), 10, 20, 40 mg/kg of the EOZC, morphine (5 mg/kg) and naloxone (2 mg/kg), respectively. Afterward, 30 min later, the formalin test was performed by intraplantar injection of formalin (50 µl, 2%). In group 8, naloxone (2 mg/kg) was injected 15 min before injection of EOZC (20 mg/kg), then 15 min later, followed by formalin. The formalin test was done as time spent for licking and biting of the injected paw. Formalin induced a biphasic pain reaction. The chemical composition of EOZC was identified using gas chromatography-mass spectrometry (GC-MS).
Results: EOZC (10, 20 and 40 mg/kg) dose dependently and morphine (5 mg/kg) reduced pain responses in the both phases of pain. (P<0.05). Naloxone (2 mg/kg) alone had no effect on the severity of pain (P>0.05) but pretreatment with naloxone inhibited EOZC-induced antinociception activity (P<0.05). In addition, according to results of GC-MS analysis, carvacrol (65.22%), thymol (19.51%), p-Cymene (4.86%) and γ-terpinene (4.63%) were the main components of the EOZC.    
Conclusion: These results demonstrate that EOZC has antinociceptive effect and this effect might mediate via opioidergic pathways.
Type of Study: Original | Subject: Behavioral Neuroscience
Received: 2017/11/6 | Accepted: 2018/01/31 | Published: 2018/04/23

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